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Related blog: CUT&Tag DNA Library Yield: What to Do if Yours Is Too Low to Detect with an Agilent Bioanalyzer or TapeStation System
What is the expected yield of a CUT&Tag DNA library?
増幅されたCUT&Tag DNAライブラリーの予想収量は、使用するDNA定量システムによって様々です。
- If you are using a NanoDrop Spectrophotometer or QIAxpert System, the typical reading is between 10-20 ng/µL for histone targets and 5-12 ng/µL for non-histone targets. If the library concentration is lower than 3 ng/µL with the NanoDrop or QIAxpert System, please refer to the CUT&Tag Troubleshooting Guide before sequencing your samples.
- If you are using a Qubit Fluorometric Quantification System or PicoGreen dsDNA Assay, the typical reading is between 3-10 ng/µL for histone targets and could be lower than 1 ng/µL for non-histone targets.
Related blog: CUT&Tag DNA Library Yield: What to Do if Yours Is Too Low to Detect with an Agilent Bioanalyzer or TapeStation System
Last updated: 2024 年 4 月 30 日
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