MAP Kinase Signaling
Phospho-ATF-2 (Thr69/71) Blocking Peptide |
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| CSTコード | 包装 | 希望納入価格 (円) |
国内在庫  2012年2月9日15時20分 現在 |
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| #1040S | 100 μg | 16,000 | ログインすると国内在庫状況がご確認いただけます。
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| 用途 | |
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| ウエスタンブロッティング、免疫組織染色 |
| 貯法 | |
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| -20℃ |
| 使用目的 | |
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| Phospho-ATF-2 (Thr69/71) Antibody （#9225）の反応をブロックし、抗体の反応特異性を確認するために使用 |
| 社内データ |
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Western Blotting
Western blot analysis of whole cell lysates from NIH/3T3 cells treated and untreated with UV light, probed with Phospho-ATF-2 (Thr69/71) Antibody #9225 (left) and with the same antibody co-incubated with specific blocking peptide (right).
IHC-P (paraffin)
Immunohistochemical staining of paraffin-embedded human breast carcinoma, using Phospho-ATF-2 (Thr69/71) Antibody #9225 preincubated with irrelevant control peptide (left) and Phospho-ATF-2 (Thr69/71) Blocking Peptide (right).
| バックグラウンド |
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The transcription factor ATF-2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins (1). ATF-2 interacts with a variety of viral oncoproteins and cellular tumor suppressors and is a target of the SAPK/JNK and p38 MAP kinase signaling pathways (2-4). Various forms of cellular stress, including genotoxic agents, inflammatory cytokines, and UV irradiation, stimulate the transcriptional activity of ATF-2. Cellular stress activates ATF-2 by phosphorylation of Thr69 and Thr71 (2-4). Both SAPK and p38 MAPK have been shown to phosphorylate ATF-2 at these sites in vitro and in cells transfected with ATF-2. Mutations of these sites result in the loss of stress-induced transcription by ATF-2 (2-4). In addition, mutations at these sites reduce the ability of E1A and Rb to stimulate gene expression via ATF-2 (2).
| 使用例 | |
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| 製品をご使用いただいて研究を発表されましたら、ぜひお知らせください。 |
| 関連製品 | |
|---|---|
| 5112 Phospho-ATF-2 (Thr71) (11G2) Rabbit mAb | |
| 9221 Phospho-ATF-2 (Thr71) Antibody | |
| 9223 ATF-2 Control Cell Extracts | |
| 9225 Phospho-ATF-2 (Thr69/71) Antibody | |
| 9226 ATF-2 (20F1) Rabbit mAb |
本製品は試験研究用です。