Jak/Stat Pathway
Stat1 Blocking Peptide (9175 Specific) |
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| CSTコード | 包装 | 希望納入価格 (円) |
国内在庫  2012年2月9日11時30分 現在 |
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| #1079S | 100 μg | 16,000 | ログインすると国内在庫状況がご確認いただけます。
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| 用途 | |
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| ウエスタンブロッティング、免疫組織染色 |
| 貯法 | |
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| -20℃ |
| 使用目的 | |
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| Stat1 (42H3) Rabbit mAb （#9175）の反応をブロックし、抗体の反応特異性を確認するために使用 |
| 社内データ |
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Western Blotting
Western blot analysis of extracts from serum starved HeLa cells, untreated or IFN-alpha-treated (100 ng/ml for 5 mintues), using Stat1 (42H3) Rabbit mAb #9175 preincubated with an unrelated control peptide (left) or Stat1 Blocking Peptide (#9175 specific) #1079 (right).
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Stat1 (42H3) Rabbit mAb (Human Specific) #9175 in the presence of control peptide (left) or Stat1 Blocking Peptide (9175 Specific) (right).
| バックグラウンド |
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The Stat1 transcription factor is activated in response to a large number of ligands (1) and is essential for responsiveness to IFN-α and IFN-γ (2,3). Phosphorylation of Stat1 at Tyr701 induces Stat1 dimerization, nuclear translocation, and DNA binding (4). Stat1 protein exists as a pair of isoforms, Stat1α (91 kDa) and the splice variant Stat1β (84 kDa). In most cells, both isoforms are activated by IFN-α, but only Stat1α is activated by IFN-γ. The inappropriate activation of Stat1 occurs in many tumors (5). In addition to tyrosine phosphorylation, Stat1 is also phosphorylated at Ser727 through a p38 mitogen-activated protein kinase (MAPK)-dependent pathway in response to IFN-α and other cellular stresses (6). Serine phosphorylation may be required for the maximal induction of Stat1-mediated gene activation.
| 使用例 | |
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| 製品をご使用いただいて研究を発表されましたら、ぜひお知らせください。 |
| 関連製品 | |
|---|---|
| 8105 SignalSlide® Phospho-Stat1/3/5 IHC Controls | |
| 9175 Stat1 (42H3) Rabbit mAb |
本製品は試験研究用です。