MAP Kinase Signaling
Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Blocking Peptide |
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| CSTコード | 包装 | 希望納入価格 (円) |
国内在庫  2012年2月9日11時30分 現在 |
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| #1150S | 100 μg | 16,000 | ログインすると国内在庫状況がご確認いただけます。
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| 用途 | |
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| 免疫組織染色 |
| 貯法 | |
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| -20℃ |
| 使用目的 | |
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| Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) Rabbit mAb (#4370) とPhospho-p44/42MAPK (Thr202/Tyr204) (20G11) Rabbit mAb (IHC Preferred) (#4376) の反応をブロックし、抗体の反応特異性を確認するために使用します。他のCST Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) 抗体や他のアプリケーションでも使用可能です。 |
| 社内データ |
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IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (20G11) Rabbit mAb #4376 preincubated with control peptide (left) or with Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Blocking Peptide (right) .
| バックグラウンド |
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Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines (1-3) and is an important target in the diagnosis and treatment of cancer (4). Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family as well as Mos and Tpl2/Cot. MEK1 and MEK2 are the primary MAPKKs in this pathway (5,6). MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK (7) and the transcription factor Elk-1 (8,9). p44/42 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases, known as DUSPs or MKPs (10), along with MEK inhibitors such as U0126 and PD98059.
- Roux, P.P. and Blenis, J. (2004) Microbiol Mol Biol Rev 68, 320-44.
- Baccarini, M. (2005) FEBS Lett 579, 3271-7.
- Meloche, S. and Pouysségur, J. (2007) Oncogene 26, 3227-39.
- Roberts, P.J. and Der, C.J. (2007) Oncogene 26, 3291-310.
- Rubinfeld, H. and Seger, R. (2005) Mol Biotechnol 31, 151-74.
- Murphy, L.O. and Blenis, J. (2006) Trends Biochem Sci 31, 268-75.
- Dalby, K.N. et al. (1998) J Biol Chem 273, 1496-505.
- Marais, R. et al. (1993) Cell 73, 381-93.
- Kortenjann, M. et al. (1994) Mol Cell Biol 14, 4815-24.
- Owens, D.M. and Keyse, S.M. (2007) Oncogene 26, 3203-13.
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本製品は試験研究用です。