Lymphocyte Signaling
Zap-70 Blocking Peptide |
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| CSTコード | 包装 | 希望納入価格 (円) |
国内在庫  2012年2月9日11時30分 現在 |
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| #1160S | 100 μg | 16,000 | ログインすると国内在庫状況がご確認いただけます。
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| 用途 | |
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| ウエスタンブロッティング、免疫組織染色 |
| 貯法 | |
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| -20℃ |
| 使用目的 | |
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| Zap-70 (99F2) Rabbit mAb (#2705)の反応をブロックし、抗体の反応特異性を確認するために使用 |
| 社内データ |
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Western Blotting
Western Blot analysis of extracts from Jurkat and Ramos cells, using Zap-70 (99F2) Rabbit mAb #2705 (left) or the same antibody preincubated with Zap-70 Blocking Peptide (right).
IHC-P (paraffin)
Immunohistochemical/immunofluorescent analysis of paraffin-embedded human tonsil, using Zap-70 (99F2) Rabbit mAb #2705 (left, green), or the same antibody preincubated with Zap-70 Blocking Peptide (right). Nuclei were counterstained using DAPI.
| バックグラウンド |
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The Syk family protein tyrosine kinase Zap-70 is expressed in T and NK cells and plays a critical role in mediating T cell activation in response to T cell receptor (TCR) engagement (1). Following TCR engagement, Zap-70 is rapidly phosphorylated on several tyrosine residues through autophosphorylation and transphosphorylation by the Src family tyrosine kinase Lck (2-6). Tyrosine phosphorylation correlates with increased Zap-70 kinase activity and downstream signaling events. Expression of Zap-70 is correlated with disease progression and survival in patients with chronic lymphocytic leukemia (7,8).
- Chu, D.H. et al. (1998) Immunol. Rev. 165, 167-180.
- Iwashima, M. et al. (1994) Science 263, 1136-1139.
- Neumeister, E.N. et al. (1995) Mol. Cell Biol. 15, 3171-3178.
- Chan, A.C. et al. (1995) EMBO J. 14, 2499-2508.
- Williams, B.L. et al. (1999) EMBO J. 18, 1832-1844.
- Di Bartolo, V. et al. (1999) J. Biol. Chem. 274, 6285-6294.
- Wiestner, A. et al. (2003) Blood 101, 4944-4951.
- Crespo, M. et al. (2003) N. Engl. J. Med. 348, 1764-1775.
| 使用例 | |
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| 製品をご使用いただいて研究を発表されましたら、ぜひお知らせください。 |
| 関連製品 | |
|---|---|
| 2701 Phospho-Zap-70 (Tyr319)/Syk (Tyr352) Antibody | |
| 2704 Phospho-Zap70 (Tyr493) Antibody | |
| 2705 Zap-70 (99F2) Rabbit mAb | |
| 2707 Zap-70 (136F12) Rabbit mAb (Alexa Fluor® 647 Conjugate) |
本製品は試験研究用です。