MAP Kinase Signaling
Phospho-p38 MAPK (Thr180/Tyr182) Blocking Peptide |
イイネ！(0) | 
|
| CSTコード | 包装 | 希望納入価格 (円) |
国内在庫  2012年2月9日15時20分 現在 |
|
|---|---|---|---|---|
| #1170S | 100 μg | 16,000 | ログインすると国内在庫状況がご確認いただけます。
|
| 用途 | |
|---|---|
| ウェスタンブロッティング、免疫組織染色 |
| 貯法 | |
|---|---|
| -20℃ |
| 使用目的 | |
|---|---|
| ウェスタンブロッティングと免疫組織染色でのPhospho-p38 MAPK (Thr180/Tyr182) (12F8) Rabbit mAb (#4631) 、ウェスタンブロッテイングでのPhospho-p38 MAP Kinase (Thr180/Tyr182) (3D7) Rabbit mAb (#9215)、 Phospho-p38 MAP Kinase (Thr180/Tyr182) (28B10) Mouse mAb （# 9216） の反応をブロックし、抗体の反応特異性を確認するために使用 |
| 社内データ |
|---|
Western Blotting
Western blot analysis of extracts from NIH/3T3 cells treated with UV, and C6 cells treated with anisomycin, using Phospho-p38 MAPK (Thr180/Tyr182) (3D7) Rabbit mAb #9215 (upper) or the same antibody preincubated with Phospho-p38 MAPK (Thr180/Tyr182) Blocking Peptide (lower).
Western Blotting
Western blot analysis of extracts from NIH/3T3 cells treated with UV, and C6 cells treated with anisomycin, using Phospho-p38 MAPK (Thr180/Tyr182) (12F8) Rabbit mAb #4631 (upper) or the same antibody preincubated with Phospho-p38 MAPK (Thr180/Tyr182) Blocking Peptide (lower).
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Phospho-p38 MAPK (Thr180/Tyr182) (12F8) Rabbit mAb #4631 preincubated with an irrelevant control peptide (left), or with Phospho-p38 MAPK (Thr180/Tyr182) Blocking Peptide (right).
| バックグラウンド |
|---|
p38 MAP kinase (MAPK), also called RK (1) or CSBP (2), is the mammalian orthologue of the yeast HOG kinase that participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAPK, p38α, β, γ (also known as Erk6 or SAPK3), and δ (also known as SAPK4) have been identified. Similar to the SAPK/JNK pathway, p38 MAPK is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharide (LPS), UV light, and growth factors (1-5). MKK3, MKK6, and SEK activate p38 MAPK by phosphorylation at Thr180 and Tyr182. Activated p38 MAPK has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6), and MEF2 (5-8).SB203580 (4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)-imidazole) is a selective inhibitor of p38 MAPK. This compound inhibits the activation of MAPKAPK-2 by p38 MAPK and subsequent phosphorylation of HSP27 (9). SB203580 inhibits p38 MAPK catalytic activity by binding to the ATP-binding pocket, but does not inhibit phosphorylation of p38 MAPK by upstream kinases (10).
- Rouse, J. et al. (1994) Cell 78, 1027-1037.
- Han, J. et al. (1994) Science 265, 808-811.
- Lee, J.C. et al. (1994) Nature 372, 739-746.
- Freshney, N.W. et al. (1994) Cell 78, 1039-1049.
- Raingeaud, J. et al. (1995) J. Biol. Chem. 270, 7420-7426.
- Zervos, A.S. et al. (1995) Proc. Natl. Acad. Sci. USA 92, 10531-10534.
- Zhao, M. et al. (1999) Mol. Cell. Biol. 19, 21-30.
- Yang, S.H. et al. (1999) Mol. Cell. Biol. 19, 4028-4038.
- Cuenda, A. et al. (1995) FEBS Lett 364, 229-33.
- Kumar, S. et al. (1999) Biochem Biophys Res Commun 263, 825-31.
| 使用例 | |
|---|---|
| 製品をご使用いただいて研究を発表されましたら、ぜひお知らせください。 |
| 関連製品 | |
|---|---|
| 4631 Phospho-p38 MAPK (Thr180/Tyr182) (12F8) Rabbit mAb |
本製品は試験研究用です。