Jak/Stat Pathway
Phospho-Stat3 (Tyr705) Blocking Peptide |
イイネ！(1) | 
|
| CSTコード | 包装 | 希望納入価格 (円) |
国内在庫  2012年2月9日11時30分 現在 |
|
|---|---|---|---|---|
| #1195S | 100 μg | 16,000 | ログインすると国内在庫状況がご確認いただけます。
|
| 用途 | |
|---|---|
| ウエスタンブロッティング、免疫組織染色 |
| 貯法 | |
|---|---|
| -20℃ |
| 使用目的 | |
|---|---|
| Phospho-Stat3 (Tyr705) (58E12) Rabbit mAb (#9135)、Phospho-Stat3 (Tyr705) Antibody (#9131) および Phospho-Stat3 (Tyr705) (3E2) Mouse mAb (#9138) の反応をブロックし、抗体の反応特異性を確認するために使用 |
| 社内データ |
|---|
Western Blotting
Western Blot analysis of extracts from serum starved HeLa cells, untreated or IFN-alpha-treated (100 ng/ml for 5 minutes), using a variety of Phospho-Stat3 and Stat 3 antibodies. The upper panels show the antibody pre-incubated with the blocking peptide while the lower panels have no blocking peptide added.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Phospho-Stat3 (Tyr705) (D3A7) Rabbit mAb #9145 in the presence of control peptide (left) or Phospho-Stat3 (Tyr705) Blocking Peptide (right).
| バックグラウンド |
|---|
The Stat3 transcription factor is an important signaling molecule for many cytokines and growth factor receptors (1) and is required for murine fetal development (2). Stat3 is constitutively activated in a number of human tumors (3,4) and possesses oncogenic potential (5) and anti-apoptotic activities (3). Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation, and DNA binding (6,7). Transcriptional activation seems to be regulated by phosphorylation at Ser727 through the MAPK or mTOR pathways (8,9). Stat3 isoform expression appears to reflect biological function as the relative expression levels of Stat3α (86 kDa) and Stat3β (79 kDa) depend on cell type, ligand exposure, or cell maturation stage (10). It is notable that Stat3β lacks the serine phosphorylation site within the carboxy-terminal transcriptional activation domain (8).
- Heim, M.H. (2001) J Recept Signal Transduct Res 19, 75-120.
- Takeda, K. et al. (1997) Proc Natl Acad Sci U S A 94, 3801-4.
- Catlett-Falcone, R. et al. (1999) Immunity 10, 105-15.
- Garcia, R. and Jove, R. (1998) J Biomed Sci 5, 79-85.
- Bromberg, J.F. et al. (1999) Cell 98, 295-303.
- Darnell, J.E. et al. (1994) Science 264, 1415-21.
- Ihle, J.N. (1995) Nature 377, 591-4.
- Wen, Z. et al. (1995) Cell 82, 241-50.
- Yokogami, K. et al. (2000) Curr Biol 10, 47-50.
- Biethahn, S. et al. (1999) Exp Hematol 27, 885-94.
| 使用例 | |
|---|---|
| 製品をご使用いただいて研究を発表されましたら、ぜひお知らせください。 |
本製品は試験研究用です。