MAP Kinase Signaling
Phospho-MEK1/2 (Ser221) Blocking Peptide |
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| CSTコード | 包装 | 希望納入価格 (円) |
国内在庫  2012年2月9日15時20分 現在 |
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| #1210S | 100 μg | 16,000 | ログインすると国内在庫状況がご確認いただけます。
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| 用途 | |
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| ウエスタンブロッティング、免疫組織染色 |
| 貯法 | |
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| -20℃ |
| 使用目的 | |
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| Phospho-MEK1/2 (Ser221) (166F8) Rabbit mAb (#2338) の反応をブロックし、抗体の反応特異性を確認するために使用 |
| 社内データ |
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Western Blotting
Western Blot analysis of extracts from NIH/3T3 cells, untreated or serum-treated, using Phospho-MEK1/2 (Ser221) (166F8) Rabbit mAb #2338 (left) or same antibody preincubated with Phospho-MEK1/2 (Ser221) Blocking Peptide (right)
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Phospho-MEK1/2 (Ser221) (166F8) Rabbit mAb #2338 in the presence of control peptide (left) or Phospho-MEK1/2 (Ser221) Blocking Peptide (right).
| バックグラウンド |
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MEK1 and MEK2, also called MAPK or Erk kinases, are dual-specificity protein kinases that function in a mitogen activated protein kinase cascade controlling cell growth and differentiation (1-3). Activation of MEK1 and MEK2 occurs through phosphorylation of two serine residues at positions 217 and 221, located in the activation loop of subdomain VIII, by Raf-like molecules. MEK1/2 is activated by a wide variety of growth factors and cytokines and also by membrane depolarization and calcium influx (1-4). Constitutively active forms of MEK1/2 are sufficient for the transformation of NIH/3T3 cells or the differentiation of PC-12 cells (4). MEK activates p44 and p42 MAP kinase by phosphorylating both threonine and tyrosine residues at sites located within the activation loop of kinase subdomain VIII.
| 使用例 | |
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| 製品をご使用いただいて研究を発表されましたら、ぜひお知らせください。 |
| 関連製品 | |
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| 2338 Phospho-MEK1/2 (Ser221) (166F8) Rabbit mAb |
本製品は試験研究用です。