Translational Control
Phospho-S6 Ribosomal Protein (Ser235/236) Blocking Peptide |
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| CSTコード | 包装 | 希望納入価格 (円) |
国内在庫  2012年2月9日15時20分 現在 |
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| #1220S | 100 μg | 16,000 | ログインすると国内在庫状況がご確認いただけます。
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| 用途 | |
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| ウェスタンブロッティング、免疫組織染色 |
| 貯法 | |
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| -20℃ |
| 使用目的 | |
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| Phospho-S6 Ribosomal Protein (Ser235/236) (91B2) Rabbit mAb (IHC Preferred) （#4857） およびPhospho-S6 Ribosomal Protein (Ser235/236) Antibody （#2211）、Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb （#4858）、 Phospho-S6 Ribosomal Protein (Ser235/236) (M20B19) Mouse mAb （#5006） の反応をブロックし、抗体の反応特異性を確認するために使用。 |
| 社内データ |
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IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Phospho-S6 Ribosomal Protein (Ser235/236) (91B2) Rabbit mAb #4857 in the presence of control peptide (left) or Phospho-S6 Ribosomal Protein (Ser235/236) Blocking Peptide (right).
Western blot analysis of extracts from 3T3 cells, untreated or treated with 20% serum, using Phospho-S6 Ribosomal Protein (Ser235/236) Antibody #2211 (left), or the same antibody incubated with specific blocking peptide (right).
| バックグラウンド |
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One way that growth factors and mitogens effectively promote sustained cell growth and proliferation is by upregulating mRNA translation (1,2). Growth factors and mitogens induce the activation of p70 S6 kinase and the subsequent phosphorylation of the S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation of mRNA transcripts that contain an oligopyrimidine tract in their 5' untranslated regions (2). These particular mRNA transcripts (5'TOP) encode proteins involved in cell cycle progression as well as ribosomal proteins and elongation factors necessary for translation (2,3). Important S6 ribosomal protein phosphorylation sites include several residues (Ser235, Ser236, Ser240, and Ser244) located within a small, carboxy-terminal region of the S6 protein (4,5).
No product specific background information is currently available for this product.
- Dufner, A. and Thomas, G. (1999) Exp. Cell Res. 253, 100-109.
- Peterson, R.T. and Schreiber, S.L. (1998) Curr. Biol. 8, R248-R250.
- Jefferies, H.B. et al. (1997) EMBO J. 16, 3693-3704.
- Ferrari, S. et al. (1991) J. Biol. Chem. 266, 22770-22775.
- Flotow, H. and Thomas, G. (1992) J. Biol. Chem. 267, 3074-3078.
| 使用例 | |
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| 製品をご使用いただいて研究を発表されましたら、ぜひお知らせください。 |
| 関連製品 | |
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| 2211 Phospho-S6 Ribosomal Protein (Ser235/236) Antibody | |
| 4857 Phospho-S6 Ribosomal Protein (Ser235/236) (91B2) Rabbit mAb | |
| 4858 Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb |
本製品は試験研究用です。