PI3K / Akt Signaling
PTEN Blocking Peptide |
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| CSTコード | 包装 | 希望納入価格 (円) |
国内在庫  2012年2月9日15時20分 現在 |
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| #1250S | 100 μg | 16,000 | ログインすると国内在庫状況がご確認いただけます。
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| 用途 | |
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| 免疫組織染色 |
| 貯法 | |
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| -20℃ |
| 使用目的 | |
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| PTEN (138G8) Rabbit mAb (#9559) の反応をブロックし、抗体の反応特異性を確認するために使用 |
| 社内データ |
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Western Blotting
Western blot analysis of extracts from COS-7 and HeLa cells using PTEN (138G6) Rabbit mAb # 9559 or the same antibody preincubated with PTEN Blocking Peptide.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using PTEN (138G8) Rabbit mAb #9559 preincubated with a control peptide (left) or with PTEN Blocking Peptide (right).
| バックグラウンド |
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PTEN (phosphatase and tensin homologue deleted on chromosome ten), also referred to as MMAC (mutated in multiple advanced cancers) phosphatase, is a tumor suppressor implicated in a wide variety of human cancers (1). PTEN encodes a 403 amino acid polypeptide originally described as a dual-specificity protein phosphatase (2). The main substrates of PTEN are inositol phospholipids generated by the activation of the phosphoinositide 3-kinase (PI3K) (3). PTEN is a major negative regulator of the PI3K/Akt signaling pathway (1,4,5). PTEN possesses a carboxy-terminal, noncatalytic regulatory domain with three phosphorylation sites (Ser380, Thr382, and Thr383) that regulate PTEN stability and may affect its biological activity (6,7). PTEN regulates p53 protein levels and activity (8) and is involved in G protein-coupled signaling during chemotaxis (9,10).
Applications: Use as a blocking reagent to evaluate the specificity of antibody reactivity in immunohistochemistry protocols and for western immunoblot analysis.Quality Control: The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry. The peptide blocks PTEN (138G8) Rabbit mAb # 9559 signal in immunohistochemistry.Directions for Use: For immunohistochemistry, add twice the volume of peptide as volume of antibody used in 100 ul total volume. Incubate for a minimum of 30 minutes prior to adding the entire volume to the slide. Recommended antibody dilutions can be found on the PTEN (138G6) Rabbit mAb #9559 data sheet.
- Cantley, L.C. and Neel, B.G. (1999) Proc Natl Acad Sci USA 96, 4240-5.
- Myers, M.P. et al. (1997) Proc Natl Acad Sci USA 94, 9052-7.
- Myers, M.P. et al. (1998) Proc Natl Acad Sci USA 95, 13513-8.
- Wan, X. and Helman, L.J. (2003) Oncogene 22, 8205-11.
- Wu, X. et al. (1998) Proc Natl Acad Sci USA 95, 15587-91.
- Vazquez, F. et al. (2000) Mol Cell Biol 20, 5010-8.
- Torres, J. and Pulido, R. (2001) J Biol Chem 276, 993-8.
- Freeman, D.J. et al. (2003) Cancer Cell 3, 117-30.
- Funamoto, S. et al. (2002) Cell 109, 611-23.
- Iijima, M. and Devreotes, P. (2002) Cell 109, 599-610.
| 使用例 | |
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| 製品をご使用いただいて研究を発表されましたら、ぜひお知らせください。 |
| 関連製品 | |
|---|---|
| 8106 SignalSlide® PTEN IHC Controls | |
| 9188 PTEN (D4.3) XP® Rabbit mAb | |
| 9559 PTEN (138G6) Rabbit mAb |
本製品は試験研究用です。