Tyrosine Kinases / Adaptors

Met, a high affinity tyrosine kinase receptor for hepatocyte growth factor (HGF, also known as scatter factor) is a disulfide-linked heterodimer made of 45 kDa α- and 145 kDa β-subunits (1,2). The α-subunit and the amino-terminal region of the β-subunit form the extracellular domain. The remainder of the β-chain spans the plasma membrane and contains a cytoplasmic region with tyrosine kinase activity. Interaction of Met with HGF results in autophosphorylation at multiple tyrosines, which recruit several downstream signaling components, including Gab1, c-Cbl and PI3 kinase (3). These fundamental events are important for all of the biological functions involving Met kinase activity. The addition of a phosphate at cytoplasmic Tyr1003 is essential for Met protein ubiquitination and degradation (4). Phosphorylation of Tyr1234/1235 in the Met kinase domain is critical to kinase activation. Phosphorylation of Tyr1349 in the Met cytoplasmic domain provides a direct binding site for Gab1 (5). Altered Met levels and/or tyrosine kinase activities are found in several types of tumors, including renal, colon and breast. Thus, Met is an attractive cancer therapeutic and diagnostic target (6).

1. Weidner, K.M. et al. (1993) J. Cell Biol. 121, 145-154.
2. Park, M. et al. (1986) Cell 45, 895-904.
3. Bardelli, A. et al. (1997) Oncogene 15, 3103-3111.
4. Taher, T.E. et al. (2002) J. Immunol. 169, 3793-3800.
5. Schaeper, U. et al. (2000) J. Cell Biol. 149, 1419-1432.
6. Traxler, P. et al. (2001) Med. Res. Rev. 21, 499-512.

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Phospho-Met (Tyr1234/1235) Blocking Peptide

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