Phosphatases
| CSTコード |
包装 |
希望納入価格 (円) |
国内在庫  |
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| #2007S | 100 μL | 57,000 | |
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SHIP2抗体製品一覧
2007 の推奨プロトコール
最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。
注：各製品に最適化されたプロトコールをリンクしています。
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2007:
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Western Blotting
| 用途 (希釈倍率) | |
| ウェスタンブロッティング (1:1,000) |
| 特異性・感度 | |
| 内在性レベルのTyr1135 がリン酸化されたSHIP2 タンパク質を検出します。 |
| 使用抗原 | |
| ヒトのSHIP2 タンパク質のTyr1135 周辺領域 (合成リン酸化ペプチド) |
Western Blotting
Western blot analysis of extracts from K562 cells, untreated or treated with λ phosphatase, using Phospho-SHIP2 (Tyr1135) Antibody (upper) or total SHIP2 (C76A7) Rabbit mAb #2839 (lower).
SH2-containing inositol phosphatase 1 (SHIP1) is a hematopoietic phosphatase that hydrolyzes phosphatidylinositol-3,4,5-triphosphate to phosphatidylinositol-3,4-bisphosphate (1). SHIP1 is a cytosolic phosphatase with an SH2 domain in its amino terminus and two NPXY Shc binding motifs in its carboxy terminus (1,2). Upon receptor cross-linking, SHIP is first recruited to the membrane junction through binding of its SH2 domain to the phospho-tyrosine in the ITIM motif (2), followed by tyrosine phosphorylation on the NPXY motif (2). The membrane relocalization and phosphorylation on the NPXY motif is essential for the regulatory function of SHIP1 (3-5). Its effect on calcium flux, cell survival, growth, cell cycle arrest and apoptosis is mediated through the PI3K and Akt pathways (3-5). Tyr1021 is located in one of the NPXY motifs in SHIP1, and its phosphorylation is important for SHIP1 function (6).
SHIP2, a homolog of SHIP1, is highly expressed in heart, skeletal muscle and placenta (7). SHIP2 negatively regulates insulin signaling (8) and polymorphisms in SHIP2 have been linked to hyperglycemia (9). Recent studies also suggest SHIP2 as a therapeutic target for the treatment of both obesity and type 2 diabetes (10,11). Tyr1135 is phosphorylated in human cancer cells (12-15).
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Tridandapani, S. et al. (1997) Mol Cell Biol 17, 4305-11.
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Liu, L. et al. (1997) J Biol Chem 272, 8983-8.
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Malbec, O. et al. (2001) J Biol Chem 276, 30381-91.
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Carver, D.J. et al. (2000) Blood 96, 1449-56.
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Scharenberg, A.M. et al. (1998) EMBO J 17, 1961-72.
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Sattler, M. et al. (2001) J Biol Chem 276, 2451-8.
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Pesesse, X. et al. (1997) Biochem Biophys Res Commun 239, 697-700.
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Wada, T. et al. (2001) Mol Cell Biol 21, 1633-46.
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Ishida, S. et al. (2006) Pancreas 33, 63-7.
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Sasaoka, T. et al. (2006) Pharmacol Ther 112, 799-809.
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Liang, X. et al. (2006) Proteomics 6, 4554-64.
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Dyson, J.M. et al. (2005) Int J Biochem Cell Biol 37, 2260-5.
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Goss, V.L. et al. (2006) Blood 107, 4888-97.
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Guo, A. et al. (2008) Proc Natl Acad Sci USA 105, 692-7.
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Rikova, K. et al. (2007) Cell 131, 1190-203.
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