Jak/Stat Pathway
Cleaved IL-1β (Asp116) Antibody
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| CSTコード |
包装 |
希望納入価格 (円) |
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| #2021S | 100 μL | 57,000 | |
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IL-1 beta抗体製品一覧
2021 の推奨プロトコール
最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。
注：各製品に最適化されたプロトコールをリンクしています。
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2021:
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Western Blotting
| 用途（希釈倍率） | |
| ウエスタンブロッティング(1:1,000) |
| 特異性・感度 | |
| 内在性レベルの17k Da のIL-1β タンパク質開裂断片を検出します。全長IL-1βタンパク質は検出しません。 |
| 使用抗原 | |
| 17k Da のIL-1β タンパク質開裂断片のN末端配列（合成ペプチド） |
Western Blotting
Western blot analysis of extracts (lanes 1 and 2) and supernatants (lanes 3 and 4) from THP-1 cells, untreated or lipopolysaccharide (LPS)-treated (10 ug/ml for 24 hours), using Cleaved IL-1beta (Asp116) Antibody (upper) or IL-1beta Antibody (lower). Cells were differentiated with 0.5 uM TPA for 3 hours. Cells were washed and plated onto 24-well plates at a density of 4 x 105 cells per well and left to adhere overnight prior to LPS treatment with the addition of 5 mM ATP for 1 hour to stimulate release of IL-1 beta.
Interleukin-1beta (IL-1beta), one of the major caspase-1 targets, is a multifunctional cytokine that is involved in a host of immune and proinflammatory responses (1). It is produced primarily by activated monocytes and macrophages. It signals through various adaptor proteins and kinases that lead to activation of numerous downstream targets (2-6). Human IL-1beta is synthesized as a 31 kDa precursor. To gain activity, the precursor must be cleaved by caspase-1 between Asp116 and Ala117 to yield a 17 kDa mature form (7,8). Detection of the 17 kDa mature form of IL-1beta is a good indicator of caspase-1 activity.
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Dinarello, C.A. (1998) Int. Rev. Immunol. 16, 457-499.
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Burns, K. et al. (1998) J. Biol. Chem. 273, 12203-12209.
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Cao, Z. et al. (1996) Nature 383, 443-446.
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Cao, Z. et al. (1996) Science 271, 1128-1131.
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Wesche, H. et al. (1997) Immunity 7, 837-847.
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Ninomiya-Tsuji, J. et al. (1999) Nature 398, 252-256.
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Thornberry, N. A. et al. (1992) Nature 356, 768-774.
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Cerretti, D. P. et al. (1992) Science 256, 97-100.
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Grahames, C.B. et al. (1999) Br J Pharmacol 127, 1915-21.
- Martinon, F. et al. (2002) The Inflammasome: A Molecular Platform Triggering Activation Of Inflammatory Caspases and processing of proIL-beta. Molecular Cell 10, 417-426. Applications: Western Blotting
- Lee, J. et al. (2011) Oncogene Applications: Western Blotting
PhosphoSitePlus®：
IL1B