Phosphatases
- Phosphatases
PhosphoSitePlus®：
PPP2CA
- 推奨プロトコール
PP2A C Subunit Antibody |
イイネ！(1) | 
|
| CSTコード | 包装 | 希望納入価格 (円) |
国内在庫  2012年2月9日11時30分 現在 |
|
|---|---|---|---|---|
| #2038S | 100 μL | 46,000 | ログインすると国内在庫状況がご確認いただけます。
|
|
2038 の推奨プロトコール 最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。 注：各製品に最適化されたプロトコールをリンクしています。 |
|---|
下記ステップについては、データシートの右側もあわせてご参照ください。
IHC-P: 抗体希釈液 / 抗原賦活化
| 用途 (希釈倍率) | |
|---|---|
| ウェスタンブロッティング (1:1,000)、免疫沈降 (1:100)、免疫組織染色 (パラフィン) (1:100)、免疫蛍光細胞染色 (IF-IC) (1:50)、フローサイトメトリー (1:100) |
| 種交差性 | |
|---|---|
| ヒト、マウス、ラット、サル、キイロショウジョウバエ、(ブタ、ニワトリ) |
| 特異性・感度 | |
|---|---|
| 内在性レベルのPP2A Catalytic Subunit タンパク質 (αおよびβアイソフォーム) を検出します。他のPP2A サブユニットタンパク質とは交差しません。 |
| 検出タンパク質の分子量 | |
|---|---|
| 36 kDa、38 kDa |
| 使用抗原 | |
|---|---|
| ヒトのPP2A Catalytic Subunit タンパク質のC末端領域のアミノ酸 (合成ペプチド) |
| 抗体の由来 | |
|---|---|
| ウサギ |
| 貯法 | |
|---|---|
| -20℃ |
| ※括弧付きの動物種は、配列が100%相同であるため反応すると推定されます。 |
| 社内データ |
|---|
Western Blotting
Western blot analysis of extracts from HeLa, NIH/3T3, C6 and COS cells using PP2A C Subunit Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using PP2A C Subunit Anitbody in the presence of control peptide (left) or PP2A C Subunit Blocking Peptide, #1067 (right).
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing cytoplasmic localization, using PP2A C Subunit Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using PP2A C Subunit Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using PP2A C Subunit Antibody in the presence of control peptide (left) or antigen-specific peptide, #1067 (right).
Flow Cytometry
Flow cytometric analysis of NIH/3T3 cells using PP2A C Subunit Antibody (blue) compared to a non-specifc negative control antibody (red).
IF-IC
Immunofluorescent analysis of paraformaldehyde fixed HeLa cells using PP2A C Subunit Antibody.
| バックグラウンド |
|---|
Protein phosphatase type 2A (PP2A) is an essential protein serine/threonine phosphatase that is conserved in all eukaryotes. PP2A is a key enzyme within various signal transduction pathways as it regulates fundamental cellular activities such as DNA replication, transcription, translation, metabolism, cell cycle progression, cell division, apoptosis and development (1-3). The core enzyme consists of catalytic C and regulatory A (or PR65) subunits, with each subunit represented by α and β isoforms (1). Additional regulatory subunits belong to four different families of unrelated proteins. Both the B (or PR55) and B' regulatory protein families contain α, β, γ and δ isoforms, with the B' family also including an ε protein. B'' family proteins include PR72, PR130, PR59 and PR48 isoforms, while striatin (PR110) and SG2NA (PR93) are both members of the B''' regulatory protein family. These B subunits competitively bind to a shared binding site on the core A subunit (1). This variable array of holoenzyme components, particularly regulatory B subunits, allows PP2A to act in a diverse set of functions. PP2A function is regulated by expression, localization, holoenzyme composition and post-translational modification. Phosphorylation of PP2A at Tyr307 by Src occurs in response to EGF or insulin and results in a substantial reduction of PP2A activity (4). Reversible methylation on the carboxyl group of Leu309 of PP2A has been observed (5,6). Methylation alters the conformation of PP2A, as well as its localization and association with B regulatory subunits (6-8).
- Janssens, V. and Goris, J. (2001) Biochem. J. 353, 417-439.
- Zolnierowicz, S. (2000) Biochem. Pharmacol. 60, 1225-1235.
- Milward, T.A. et al. (1999) Trends Biochem. Sci. 24, 186-191.
- Chen, J. et al. (1992) Science 257, 1261-1264.
- Turowski, P. et al. (1995) J. Cell. Biol. 129, 397-410.
- Lee, J. et al. (1996) Proc. Natl. Acad. Sci. USA 93, 6043-6047.
- Tolstykh, T. et al. (2000) EMBO J. 19, 5682-5691.
- Yu, X.X. et al. (2001) Mol. Biol. Cell 12, 185-199.
| 使用例 | |
|---|---|
| 製品をご使用いただいて研究を発表されましたら、ぜひお知らせください。 |
本製品は試験研究用です。