Chromatin Regulation / Acetylation
| CSTコード |
包装 |
希望納入価格（円） |
国内在庫  |
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| #2047S | 100 μL | 46,000 | |
|
CENPA抗体製品一覧
推奨プロトコール
最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。
注：各製品に最適化されたプロトコールをリンクしています。
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2047:
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Immunoprecipitation
Western Blotting
Western Blotting
| 用途（希釈倍率） | |
| ウエスタンブロッティング（1:1,000）、 免疫沈降（1:50） |
| 特異性・感度 | |
| 内在性レベルのマウスのCENP-A タンパク質を検出します。Histone H3 などの他のHistone タンパク質とは交差しません。 |
| 使用抗原 | |
| マウスのCENP-A タンパク質のN末端周辺領域（合成ペプチド） |
Western Blotting
Western blot analysis of cell lysates from NIH/3T3 and C2C12 cells using CENP-A (C5H3) Rabbit mAb.
Modulation of chromatin structure plays a critical role in the regulation of transcription and replication of the eukaryotic genome. The nucleosome, made up of four core histone proteins (H2A, H2B, H3, and H4), is the primary building block of chromatin. In addition to the growing number of post-translational histone modifications regulating chromatin structure, cells can also exchange canonical histones with variant histones that can directly or indirectly modulate chromatin structure (1). CENP-A, also known as the chromatin-associated protein CSE4 (capping-enzyme suppressor 4-p), is an essential histone H3 variant that replaces canonical histone H3 in centromeric heterochromatin (2). The greatest divergence between CENP-A and canonical histone H3 occurs in the amino-terminal tail of the protein, which binds linker DNA between nucleosomes and facilitates proper folding of centromeric heterochromatin (3). The amino-terminal tail of CENP-A is also required for recruitment of other centromeric proteins (CENP-C, hSMC1, hZW10), proper kinetochore assembly and chromosome segregation during mitosis (4). Additional sequence divergence in the histone fold domain is responsible for correct targeting of CENP-A to the centromere (5). Many of the functions of CENP-A are regulated by phosphorylation (6,7). Aurora A-dependent phosphorylation of CENP-A on Ser7 during prophase is required for proper targeting of Aurora B to the inner centromere in prometaphase, proper kinetochore/microtubule attachment and proper alignment of chromosomes during mitosis (6).
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Jin, J. et al. (2005)
Trends Biochem Sci
30, 680-7.
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Ausió, J. (2006)
Brief Funct Genomic Proteomic
5, 228-43.
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Heit, R. et al. (2006)
Biochem Cell Biol
84, 605-18.
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Van Hooser, A.A. et al. (2001)
J Cell Sci
114, 3529-42.
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Black, B.E. et al. (2004)
Nature
430, 578-82.
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Kunitoku, N. et al. (2003)
Dev Cell
5, 853-64.
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Zeitlin, S.G. et al. (2001)
J Cell Biol
155, 1147-57.
