Cytoskeletal Signaling
Mena Antibody
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| CSTコード |
包装 |
希望納入価格 (円) |
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| #2075S | 100 μL | 46,000 | |
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Mena抗体製品一覧
2075 の推奨プロトコール
最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。
注：各製品に最適化されたプロトコールをリンクしています。
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2075:
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Western Blotting
| 用途 (希釈倍率) | |
| ウェスタンブロッティング (1:1,000) |
| 特異性・感度 | |
| 内在性レベルのMena タンパク質を検出します。 |
| 検出タンパク質の分子量 | |
| 80 kDa、88 kDa、140 kDa |
| 使用抗原 | |
| ヒトのMena タンパク質 (合成ペプチド) |
Western Blotting
Western blot analysis of extracts from C6 and A431 cells using Mena Antibody.
Mena (mammalian enabled), EVL, and VASP are members of the Ena/VASP family, which is involved in controlling cell shape and cell movement by shielding actin filaments from capping proteins (1). Ena/VASP proteins have three specific domains: an amino-terminal EVH1 domain controlling protein localization; a central proline-rich domain mediating interactions with both SH3 and WW domain containing proteins, including profilin; and a carboxy-terminal domain causing tetramerization and binding to actin (2). Mena interacts with actin filaments at the growing ends localizing to lamellipodia and to tips of growth cone filopodia in neurons. Axons projecting from interhemispheric cortico-cortical neurons are misrouted in newborn, homozygous Mena knock-out mice (3). Mena is phosphorylated at Ser236 by PKA, thereby promoting filopodial formation and elongation in the growth cone (4).Three forms of Mena corresponding to 80, 88 and 140 kD are known. The 80 kD protein is broadly expressed in contrast to the 140 kD protein which is enriched in neural cell types. Alternative splicing produces these forms. The 88 kD protein is mainly found in embryonic cell types and is likely the result of post-translational modification. Expression of all three forms is completely eliminated in Mena homozygous mutant animals (1, 3).
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Gertler, F.B. et al. (1996) Cell 87, 227-39.
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Small, J.V. (2008) Nat Cell Biol 10, 118-20.
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Lanier, L.M. et al. (1999) Neuron 22, 313-25.
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Lebrand, C. et al. (2004) Neuron 42, 37-49.
PhosphoSitePlus®：
Mena