Tyrosine Kinases / Adaptors
Phospho-Src Family (Tyr416) Antibody
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イイネ！(1)
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| CSTコード |
包装 |
希望納入価格 (円) |
国内在庫  |
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| #2101S | 100 μL | 57,000 | |
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| #2101L | 300 μL | 137,000 | |
Src抗体製品一覧
2101 の推奨プロトコール
最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。
注：各製品に最適化されたプロトコールをリンクしています。
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2101:
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Western Blotting
| 用途 (希釈倍率) | |
| ウェスタンブロッティング (1:1,000) |
| 種交差性 | |
| ヒト、マウス、ラット、(ニワトリ、アフリカツメガエル) |
| 特異性・感度 | |
| 内在性レベルのTyr416 がリン酸化されたSrc タンパク質を検出します。同等の位置がリン酸化されたSrc ファミリータンパク質 (Lyn、Fyn、Lck、Yes、Hck) と交差反応する可能性があります。Tyr527 がリン酸化されたSrc タンパク質とは交差しません。リン酸化されたRTK タンパク質とは交差反応する可能性があります。 |
| 使用抗原 | |
| ヒトのSrc タンパク質のTyr416 周辺領域 (合成リン酸化ペプチド) |
| ※括弧付きの動物種は、配列が100%相同であるため反応すると推定されます。 |
Western Blotting
Western blot analysis of extracts from serum-starved COLO 201 cells, v-Src antibody immunoprecipitates from COLO 201 cells and PDGF-treated NIH/3T3 cells, treated with lambda phosphatase as indicated, using Phospho-Src (Tyr416) Antibody (upper) or v-Src antibody (lower).
The Src family of protein tyrosine kinases, which includes Src, Lyn, Fyn, Yes, Lck, Blk, and Hck, are important in the regulation of growth and differentiation of eukaryotic cells (1). Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr527 in the carboxy-terminal tail by Csk renders the enzyme less active (2).
Lyn is a member of the Src family that is predominantly expressed in hematopoietic cells (3). Lyn participates in signaling from multiple cell surface receptors such as the B cell antigen receptor (BCR) and CD40 (4).Lck is essential for T-lymphocyte activation and differentiation (5,6). Phosphorylation of the carboxy-terminal Tyr505 downregulates Lck activity, while phosphorylation at Tyr394 leads to an increase in Lck activity (7).
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Thomas, S.M. and Brugge, J.S. (1997) Annu. Rev. Cell Dev. Biol. 13, 513-609.
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Hunter, T. (1987) Cell 49, 1-4.
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Yamanshi,, Y. et al. (1989) Proc. Natl. Acad. Sci. USA 86, 6538-6542.
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Yamanashi, Y. et al. (1991) Science 251, 192-194.
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Molina, T. J. et al. (1992) Nature 357, 161-164.
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Straus, D. B. et al. (1992) Cell 70, 585-593.
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Chow, L. M. et al. (1993) Nature 365, 156-160.
- Gonfloni, S. et al. (1999) Leucine 255 of Src couples intramolecular interactions to inhibition of catalysis. Nat. Struct. Biol. 6, 760-764. Applications: Western Blot
- Gonfloni, S. et al. (2000) Crosstalk between the catalytic and regulatory domains allows bidirectional regulation of Src. Nat. Struct. Biol. 7, 281-286. Applications: Western Blot
- Ibitayo, A. I. et al. (1998) Src kinase and PI 3-kinase as a transduction pathway in ceramide-induced contraction of colonic smooth muscle. Am. J. Physiol. 275, 705-711. Applications: Western Blot
- Wu, W. et al. (2002) Src-dependent phosphorylation of the epidermal growth factor receptor on tyrosine 845 is required for zinc-induced Ras activation. J. Biol. Chem. 277 (27), 24252-24257. Applications: Western Blotting
- Martinon, F. et al. (2002) The Inflammasome: A Molecular Platform Triggering Activation of Inflammatory Caspases and Processing of proIL-beta. Mol. Cell 10, 417-426. Applications: Western Blotting
- Nagata, Y. et al. (2004) PTEN activation contributes to tumor inhibition by trastuzumab, and loss of PTEN predicts trastuzumab resistance in patients. Cancer Cell 6, 117-127. Applications: Western Blotting
- White, D. E. et al. (2004) Targeted disruption of beta1-integrin in a transgenic mouse model of human breast cancer reveals an essential role in mammary tumor induction. Cancer Cell 6, 159-170. Applications: IC-IF Western Blotting
- Wang, D. et al. (2000) Distinct effects of N-acetylcysteine and nitricoxide on angiotensin II-induced epidermal growth factor receptor phosphorylation and intracellular Ca(2+) levels. J. Biol. Chem. 275, 12223-12230. Applications: Western Blot
- Nikolic, D.S. et al. (2011) Blood 118, 4841-52. Applications: Western Blotting
- Thelemann, A. et al. (2005) Mol Cell Proteomics 4, 356-76. Applications: Western Blotting
- Oikawa, T. et al (2008) J Cell Biol 182, 157-69. Applications: Western Blotting
