Adhesion
PhosphoSitePlus®：
P-Cadherin Antibody |
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| CSTコード | 包装 | 希望納入価格 (円) |
国内在庫  2012年2月9日15時20分 現在 |
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| #2130S | 100 μL | 46,000 | ログインすると国内在庫状況がご確認いただけます。
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2130 の推奨プロトコール 最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。 注：各製品に最適化されたプロトコールをリンクしています。 |
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| 用途 (希釈倍率) | |
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| ウェスタンブロッティング (1:1,000)、免疫沈降 (1:100)、免疫蛍光細胞染色 (IF-IC) (1:100)、フローサイトメトリー (1:25) |
| 種交差性 | |
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| ヒト |
| 特異性・感度 | |
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| 内在性レベルのP-Cadherin タンパク質を検出します。他のCadherin ファミリータンパク質とは交差しません。 |
| 検出タンパク質の分子量 | |
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| 120 kDa |
| 使用抗原 | |
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| ヒトのP-Cadherin タンパク質 (合成ペプチド) |
| 抗体の由来 | |
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| ウサギ |
| 貯法 | |
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| -20℃ |
| 社内データ |
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Western Blotting
Western blot anlaysis of extracts from A431, Panc1 and HUVE cells, using P-Cadherin Antibody.
IP
Immunoprecipitation of P-cadherin from A431 cell extracts, using P-Cadherin Antibody. Immunoblot was performed with the same antibody.
Flow Cytometry
Flow cytometric analysis of A431 cells, using P-Cadherin antibody (blue) compared to a nonspecific negative control antibody (red).
IF-IC
Immunofluorescent analysis of A431 cells, using P-Cadherin Antibody (left). Nuclei in the same cells were stained with DAPI (right).
IF-IC
Confocal immunofluorescent images of A431 cells labeled with P-Cadherin Antibody (green, left) compared to an isotype control (right). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). Blue pseudocolor = DRAQ™ (fluorescent DNA dye).
| バックグラウンド |
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Cadherins are a superfamily of transmembrane glycoproteins that contain cadherin repeats of approximately 100 residues in their extracellular domain. Cadherins mediate calcium-dependent cell-cell adhesion and play critical roles in normal tissue development (1). The classic cadherin subfamily includes N-, P-, R-, B- and E-cadherins as well as about ten other members that are found in adherens junctions, a cellular structure near the apical surface of polarized epithelial cells. The cytoplasmic domain of classical cadherins interacts with β-catenin, γ-catenin (also called plakoglobin), and p120 catenin. β-catenin and γ-catenin associate with α-catenin, which links the cadherin-catenin complex to the actin cytoskeleton (1,2). While β- and γ-catenin play structural roles in the junctional complex, p120 regulates cadherin adhesive activity and trafficking (1-4). E-cadherin is considered an active suppressor of invasion and growth of many epithelial cancers (1-3). Recent studies indicate that cancer cells have up-regulated N-cadherin in addition to loss of E-cadherin. This change in cadherin expression is called the "cadherin switch". N-cadherin cooperates with the FGF receptor, leading to overexpression of MMP-9 and cellular invasion (3). In endothelial cells, VE-cadherin signaling, expression, and localization correlate with vascular permeability and tumor angiogenesis (5,6). Expression of P-cadherin, which is normally present in epithelial cells, is also altered in ovarian and other human cancers (7,8).
- Wheelock, M.J. and Johnson, K.R. (2003) Annu. Rev. Cell. Dev. Biol. 19, 207-235.
- Christofori, G. (2003) EMBO J. 22, 2318-2323.
- Hazan, R.B. et al. (2004) Ann. NY Acad. Sci. 1014, 155-163.
- Bryant, D.M. and Stow, J.L. (2004) Trends Cell Biol. 14, 427-434.
- Rabascio, C. et al. (2004) Cancer Res. 64, 4373-4377.
- Yamaoka-Tojo, M. et al. (2006) Arterioscler. Thromb. Vasc. Biol. 26, 1991-1997.
- Patel, I.S. et al. (2003) Int. J. Cancer 106, 172-177.
- Sanders, D.S. et al. (2000) J. Pathol. 190, 526-530.
| 使用例 | |
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本製品は試験研究用です。