Chromatin Regulation / Acetylation
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PhosphoSitePlus®：
| CSTコード | 包装 | 希望納入価格 (円) |
国内在庫  2012年2月3日18時20分 現在 |
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| #2139S | 100 μL | 46,000 | ログインすると国内在庫状況がご確認いただけます。
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2139 の推奨プロトコール 最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。 注：各製品に最適化されたプロトコールをリンクしています。 |
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下記ステップについては、データシートの右側もあわせてご参照ください。
IHC-P: 抗体希釈液 / 抗原賦活化
| 用途 (希釈倍率) | |
|---|---|
| ウェスタンブロッティング (1:1,000)、免疫沈降 (1:50)、免疫組織染色 (パラフィン) (1:200)、免疫蛍光細胞染色 (IF-IC) (1:400)、フローサイトメトリー (1:400) |
| 種交差性 | |
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| ヒト、マウス、ラット、サル |
| 特異性・感度 | |
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| 内在性レベルのLSD1 タンパク質を検出します。 |
| 検出タンパク質の分子量 | |
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| 110 kDa |
| 使用抗原 | |
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| ヒトのLSD1 タンパク質のN末端近傍領域 (合成ペプチド) |
| 抗体の由来 | |
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| ウサギ |
| 貯法 | |
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| -20℃ |
| 社内データ |
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Western Blotting
Western blot analysis of cell lysates from various cell lines, using LSD1 Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma showing nuclear localization, using LSD1 Antibody.
IHC-P (paraffin)
Immunohistochemical staining of paraffin-embedded human breast carcinoma, using LSD1 Antibody preincubated with control peptide (left) or antigen-specific peptide (right).
Flow Cytometry
Flow cytometric analysis of untreated HeLa cells, using LSD1 Antibody (blue) compared to a nonspecific negative control antibody (red).
IF-IC
Immunofluorescent analysis of HeLa cells, using LSD1 Antibody (right). Nuclei are stained with DAPI (left).
| バックグラウンド |
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Lysine-specific demethylase 1 (LSD1; also known as AOF2 and BHC110) is a nuclear amine oxidase homolog that acts as a histone demethylase and transcription cofactor (1). Gene activation and repression is specifically regulated by the methylation state of distinct histone protein lysine residues. For example, methylation of histone H3 at Lys4 facilitates transcriptional activation by coordinating the recruitment of BPTF, a component of the NURF chromatin remodeling complex, and WDR5, a component of multiple histone methyltransferase complexes (2,3). In contrast, methylation of histone H3 at Lys9 facilitates transcriptional repression by recruiting HP1 (4,5). LSD1 is a component of the CoREST transcriptional co-repressor complex that also contains CoREST, CtBP, HDAC1 and HDAC2. As part of this complex, LSD1 demethylates mono-methyl and di-methyl histone H3 at Lys4 through a FAD-dependent oxidation reaction to facilitate neuronal-specific gene repression in non-neuronal cells (1,6,7). In contrast, LSD1 associates with androgen receptor in human prostate cells to demethylate mono-methyl and di-methyl histone H3 at Lys9 and facilitate androgen receptor-dependent transcriptional activation (8). Therefore, depending on gene context LSD1 can function as either a transcriptional co-repressor or co-activator. LSD1 activity is inhibited by the amine oxidase inhibitors pargyline, deprenyl, clorgyline and tranylcypromine (8).
- Shi, Y. et al. (2004) Cell 119, 941-953.
- Wysocka, J. et al. (2006) Nature 442, 86-90.
- Wysocka, J. et al. (2005) Cell 121, 859-872.
- Jacobs, S.A. and Khorasanizadeh, S. (2002) Science 295, 2080-2083.
- Nielsen, P.R. et al. (2002) Nature 416, 103-107.
- Shi, Y.J. et al. (2005) Mol. Cell 19, 857-864.
- Lee, M.G. et al. (2005) Nature 437, 432-435.
- Metzger, E. et al. (2005) Nature 437, 436-439.
| 使用例 | |
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| 製品をご使用いただいて研究を発表されましたら、ぜひお知らせください。 |
本製品は試験研究用です。