Apoptosis and Autophagy
TANK Antibody
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| CSTコード |
包装 |
希望納入価格 (円) |
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| #2141S | 100 μL | 46,000 | |
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2141 の推奨プロトコール
最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。
注：各製品に最適化されたプロトコールをリンクしています。
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2141:
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Immunoprecipitation
Western Blotting
| 用途 (希釈倍率) | |
| ウェスタンブロッティング (1:1,000)、免疫沈降 (1:50) |
| 種交差性 | |
| ヒト、マウス、ラット、(サル、ウシ、イヌ) |
| 特異性・感度 | |
| 内在性レベルのTANK タンパク質を検出します。他のTRAF ファミリータンパク質とは交差しません。 |
| 使用抗原 | |
| ヒトTANK タンパク質のSer357 周辺領域 (合成ペプチド) |
| ※括弧付きの動物種は、配列が100%相同であるため反応すると推定されます。 |
Western Blotting
Western blot analysis of extracts from ACHN (human), PC-12 (rat) and A20 (mouse), using TANK Antibody.
TRAFs (TNF receptor-associated factors) are a family of multifunctional adaptor proteins that bind to surface receptors and recruit additional proteins to form multiprotein signaling complexes capable of promoting cellular responses (1-3). Members of the TRAF family share a common carboxy-terminal "TRAF domain" which mediates interactions with associated proteins; many also contain amino-terminal Zinc/RING finger motifs. The first TRAFs identified, TRAF1 and TRAF2, were found by virtue of their interactions with the cytoplasmic domain of TNF-receptor 2 (TNFRII) (4). The six known TRAFs (TRAF1-6) act as adaptor proteins for a wide range of cell surface receptors and participate in the regulation of cell survival, proliferation, differentiation, and stress responses.
TRAF-associated NF-κB activator (TANK), also known as TRAF-interacting protein (I-TRAF), is a TRAF binding protein that demonstrates both stimulatory and inhibitory properties (5,6). TANK binds to the carboxy domain of the TRAF1, -2 and -3. Overexpression of TANK prevents the association of TRAF2 with TNFR2, inhibiting TNFR2 and CD40 induced NF-κB activation (6). TANK is also reported to synergize with low levels of TRAF2 to activate NF-κB (5). TANK assists in the activation of NF-κB via association and activation of TANK-binding kinase 1 (TBK1) or IKKε which promotes activation of the IKK complex (7,8). It has also been shown that TANK may synergize with TRAF2, TRAF5, and TRAF6 but not TRAF3 in SAPK activation (9). TNFα stimulation results in IKKβ-dependent phosphorylation of TANK which may provide negative feedback regulation of TANK mediated NF-κB activation (10).
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Arch, R.H. et al. (1998) Genes Dev. 12, 2821-2830.
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Chung, J. Y. et al. (2002) J. Cell Sci. 115, 679-688.
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Bradley, J.R. and Pober, J.S. (2001) Oncogene 20, 6482-6491.
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Rothe, M. et al. (1994) Cell 78, 681-692.
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Cheng, G. and Baltimore, D. (1996) Genes Dev. 10, 963-973.
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Rothe, M. et al. (1996) Proc. Natl. Acad. Sci. USA 93, 8241-8846.
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Pomerantz, J.L. and Baltimore, D. (1999) EMBO J. 18, 6694-6704.
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Chariot, A. et al. (2002) J. Biol. Chem. 277, 37029-37036.
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Chin, A.I. et al. (1999) Mol. Cell. Biol. 19, 6665-6672.
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Bonif, M. et al. (2006) Biochem. J. 394, 593-603.
PhosphoSitePlus®：
TANK