Cytoskeletal Signaling
| CSTコード |
包装 |
希望納入価格(円) |
国内在庫  |
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| #2146 | 100 μL | 46,000 | |
|
tubulin beta-2抗体製品一覧
推奨プロトコール
最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。
注:各製品に最適化されたプロトコールをリンクしています。
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2146:
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Flow
IHC / Paraffin
Immunofluorescence
Western Blotting
| 用途(希釈倍率) | |
| ウエスタンブロッティング(1:1,000)、免疫組織染色(パラフィン)(1:50)、免疫蛍光細胞染色(IF-IC)(1:50)、フローサイトメトリー(1:25) |
| 特異性・感度 | |
| 内在性レベルのβ-Tubulin タンパク質を検出します。α-Tubulin タンパク質とは交差しません。 |
| 使用抗原 | |
| ヒトのβ-Tubulin タンパク質(合成ペプチド) |
Western Blotting
Western blot analysis of extracts from CAD and C6 cells using β-Tubulin Antibody.
Western Blotting
Western blot analysis of recombinant alpha-tubulin and beta-tubulin GST-fusion proteins and extracts from CAD cells using α-Tubulin Antibody #2144(left), β-Tubulin Antibody (middle) and GST Antibody #2622 (right).
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using β-Tubulin (9F3) Rabbit mAb #2128 in the presence of control peptide (left) or β-Tubulin Blocking Peptide (right).
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing cytoplasmic localization, using β-Tubulin Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using β-Tubulin Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human MALToma, using β-Tubulin Antibody.
Flow Cytometry
Flow cytometric analysis of C6 cells, using β-Tubulin Antibody (blue) compared to a nonspecific negative control antibody (red).
IF-IC
Confocal microscopic images of NIH/3T3 cells showing cytoskeletal stain with β-Tubulin Antibody (A) compared to an isotype control (B).
The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is necessary to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1).
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Westermann, S. and Weber, K. (2003)
Nat. Rev. Mol. Cell Biol.
4, 938 -947.