Neuroscience
| CSTコード | 包装 | 希望納入価格(円) |
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|---|---|---|---|---|
| #2148 | 100 μL | 46,000 | ログインすると国内在庫状況がご確認いただけます。
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| 用途(希釈倍率) | |
|---|---|
| ウェスタンブロッティング(1:1,000)、免疫組織染色(パラフィン)(1:50)、免疫蛍光細胞染色(IF-IC)(1:50)、フローサイトメトリー(1:50) | |
| 種交差性 | |
|---|---|
| ヒト、マウス、ラット、サル、ウシ、ゼブラフィッシュ | |
| 特異性・感度 | |
|---|---|
| 内在性レベルのα/β-Tubulin タンパク質を検出します。α/β-Tubulin 組換えタンパク質と交差します。 | |
| 検出タンパク質の分子量 | |
|---|---|
| 55 kDa | |
| 使用抗原 | |
|---|---|
| ヒトのα-Tubulin およびβ-Tubulinタンパク質(合成ペプチド) | |
| 抗体の由来 | |
|---|---|
| ウサギ | |
| 貯法 | |
|---|---|
| -20℃ | |
| 社内データ |
|---|
Western Blotting
Western blot analysis of extracts from HeLa, NIH/3T3, C6 and COS-7 cells, using α/β-Tubulin Antibody.
Western Blotting
Western blot analysis of recombinant alpha-tubulin and beta-tubulin GST-fusion proteins, and extracts from NIH/3T3 and C6 cells, using α/β-Tubulin Antibody (left) and GST Antibody #2622 (right).
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using α/β-Tubulin Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, showing cytoplasmic localization, using α/β-Tubulin Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human MALToma using α/β-Tubulin Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using β-Tubulin (9F3) Rabbit mAb #2128 in the presence of control peptide (left) or β-Tubulin Blocking Peptide (right).
Flow Cytometry
Flow cytometric analysis of C6 cells using α/β-Tubulin Antibody (blue) compared to a nonspecific negative control antibody (red).
IF-IC
Confocal microscopic images of NIH/3T3 cells showing cytoskeletal stain with α/β-Tubulin Antibody (A) compared to an isotype control (B).
IF-IC
Confocal immunofluorescent analysis of NIH-3T3 cells, using α/β-Tubulin Antibody (green) and Phospho-Histone H3 (Ser10) (6G3) Mouse mAb #9706 (red) showing different stages of the cell cycle.
| バックグラウンド |
|---|
The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is necessary to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1).
| 使用文献 |
|---|
- Marini, F. et al. (2006) Proc Natl Acad Sci U S A 103, 17325-30. Applications: Western Blotting
本製品は試験研究用です。


