Apoptosis and Autophagy
HtrA2 Antibody
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| CSTコード |
包装 |
希望納入価格 (円) |
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| #2176S | 100 μL | 46,000 | |
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2176 の推奨プロトコール
最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。
注：各製品に最適化されたプロトコールをリンクしています。
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2176:
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Western Blotting
| 用途 (希釈倍率) | |
| ウェスタンブロッティング (1:1,000) |
| 特異性・感度 | |
| 内在性レベルのHtrA2 タンパク質のMature Form を検出します。HtrA2 タンパク質のUnprocessed Form も検出すると思われます。このUnprocessed Form は、HtrA2 を過剰発現した細胞にみられます。 |
| 使用抗原 | |
| HtrA タンパク質のSer335 周辺領域 (合成ペプチド) |
| ※括弧付きの動物種は、配列が100%相同であるため反応すると推定されます。 |
Western Blotting
Western blot analysis of extracts from HT1080, Raji and BaF3 cell lines, using HtrA2 Antibody.
HtrA2 (High temperature requirement protein A2)/Omi is a serine protease with homology to the E. coli HtrA protein (DegP) and is thought to be involved in apoptosis and stress induced degradation of misfolded proteins (1). While HtrA2 was orignally identified to be present in either the nucleus (1) or endoplasmic reticulum (2), subsequent studies have since shown it to be localized in mitochondria and released during apoptosis (3-7). HtrA2 is produced as a 50 kDa zymogen that is cleaved to generate a 36 kDa mature protein that exposes an amino terminal motif (AVPS) resembling that of the IAP inhibitor Smac/Diablo (3-7). Like Smac, interaction between HtrA2 and IAP family members such as XIAP antagonizes their inhibition of caspase activity and protection from apoptosis (3-7). Interestingly, HtrA2 knock-out mice did not show show signs of reduced apoptosis, but rather had a loss of neurons in the striatum and a Parkinson's-like phenotype, suggesting that HtrA2 might have a neuroprotective function (9-11). This activity is associated with the protease activity of HtrA2 (9). Furthermore, loss of function mutations in the HtrA2 gene have been found in Parkinson's disease (12).
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Gray, C.W. et al. (2000) Eur. J. Biochem. 267, 5699-5710.
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Faccio, L. et al. (2000) J. Biol. Chem. 275, 2581-2588.
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Suzuki, Y. et al. (2001) Mol. Cell 8, 613-621.
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Hegde, R. et al. (2002) J. Biol. Chem. 277, 432-438.
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Martins, L.M. et al. (2002) J. Biol. Chem. 277, 439-444.
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van Loo, G. et al. (2002) Cell Death Differ. 9, 20-26.
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Verhagen, A.M. et al. (2002) J. Biol. Chem. 277, 445-454.
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Martins, L.M. et al. (2002) J. Biol. Chem. 277, 439-444.
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Jones, J.M. et al. (2003) Nature 425, 721-727.
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Vaux, D.L. and Silke, J. (2003) Cell 115, 251-253.
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Martins, L.M. et al. (2004) Mol. Cell Biol. 24, 9848-9862.
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Strauss, K.M. et al. (2005) Hum. Mol. Genet. 14, 2099-2111.
PhosphoSitePlus®：
HTRA2