Apoptosis and Autophagy
| CSTコード |
包装 |
希望納入価格 (円) |
国内在庫  |
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| #2182S | 100 μL | 46,000 | |
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DAP5抗体製品一覧
2182 の推奨プロトコール
最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。
注：各製品に最適化されたプロトコールをリンクしています。
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2182:
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Western Blotting
| 用途（希釈倍率） | |
| ウエスタンブロッティング(1:1,000) |
| 特異性・感度 | |
| 内在性レベルのDAP5 タンパク質を検出します。他のDAP ファミリータンパク質とは交差しません。 |
| 使用抗原 | |
| ヒトのDAP5 タンパク質の805番目のアミノ酸周辺領域（合成ペプチド） |
Western Blotting
Western blot analysis of extracts from human (Jurkat and HeLa), mouse (NIH/3T3 and L929) and rat (C6 and PC12) cells, using DAP5 Antibody.
Death associated protein 1 (DAP1) is a 15 kDa protein that functions as a positive mediator of cell death initiated by interferon-gamma (1,2). The DAP1 protein is proline rich and possesses one SH3 binding motif, as well as several consensus protein kinase phosphorylation sites (1). The protein is localized in the cytoplasm, but the detailed mechanism of its proapoptotic function is unclear. Death associated protein 3 (DAP3) is widely expressed, and the expression is upregulated during membrane receptor-mediated apoptosis. In interferon-gamma- and Fas-induced apoptosis, DAP3 acts as a positive mediator, functioning downstream of the receptor signaling complex and upstream of the effector caspases (3,4). Death associated protein 5 (DAP5) is a 97 kDa protein with a high degree of amino acid sequence homology to eukaryotic translation initiation factor 4G (Elf4G) (1,5). Compared with elF4G, DAP5 lacks the amino-terminal region necessary for cap-dependent translation, and has a unique carboxy-terminal region that functions as a regulator of interferon-gamma-induced cell death (5,6). During induction of apoptosis, DAP5 is cleaved at aspartic acid 790. The carboxy-terminal truncated form of DAP5 functions as a cap-independent translation initiation factor responsible for the mediation of its own translation during apoptosis (7).
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Deiss, L. P. et al. (1995) Genes Dev. 9, 15-30.
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Levy-Strumpf, N. and Kimchi, A. (1998) Oncogene 17, 3331-3340.
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Kissil, J. L. et al. (1995) J. Biol. Chem. 270, 27932-27936.
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Kissil, J. L. et al. (1999) EMBO J. 18, 353-362.
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Imataka, H. et al. (1997) EMBO J. 16, 817-825.
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Levy-Strumpf, N. et al. (1997) Mol. Cell. Biol. 17, 1615-1625.
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Henis-Korenblit, S. et al. (2000) Mol. Cell. Biol. 20, 496-506.
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