Chromatin Regulation / Acetylation
ESET (C1C12) Rabbit mAb
|
イイネ！(1)
| 
|
| CSTコード |
包装 |
希望納入価格 (円) |
国内在庫  |
ご登録代理店情報 カスタマー情報にご登録いただいた代理店を表示しています。
ご登録代理店の変更は こちら。 |
| #2196S | 100 μL | 46,000 | |
|
2196 の推奨プロトコール
最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。
注：各製品に最適化されたプロトコールをリンクしています。
| | |
-
2196:
-
Immunofluorescence
Immunoprecipitation
Western Blotting
| 用途（希釈倍率） | |
| ウエスタンブロッティング（1:1,000）、免疫沈降（1:50）、免疫蛍光細胞染色（IF-IC）（1:25） |
| 特異性・感度 | |
| 内在性レベルのESET タンパク質を検出します。Histone Methyltrasferase タンパク質含有の他のSET ドメインとは交差しません。 |
| 使用抗原 | |
| ヒトのESET タンパク質のC末端領域（合成ペプチド） |
Western Blotting
Western blot analysis of cell lysates from MCF7 and 293 cells using ESET (C1C12) Rabbit mAb.
IF-IC
Confocal immunofluorescent analysis of MCF-7 cells using ESET (C1C12) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red).
The Erg-associated protein with SET domain (ESET), also known as SET-domain, bifurcated 1 (SETDB1) protein, is a member of a family of histone lysine methyltransferases, each of which contains a conserved catalytic SET domain originally identified in Drosophila Su[var]3-9, Enhancer of zeste, and Trithorax proteins (1). ESET also contains tudor and methyl-CpG-binding domains, which may coordinate binding to methylated histones and methylated DNA, respectively (1). ESET methylates histone H3 Lys9, creating a transcriptionally repressive mark that facilitates gene silencing (1-3). However, unlike SUV39H histone H3 Lys9 methyltransferases, which function mainly in heterochromatin regions such as pericentric heterochromatin, ESET functions mainly in euchromatic regions to repress gene promoters (3). ESET interacts with a variety of proteins, including transcription factors (ERG), histone deacetylases (HDAC1/2), DNA methyltransferases (DNMT3A/B) and transcriptional co-repressors (mSin3A/B, MBD1, KAP-1, the ATFa-associated modulator mAM) (1-6). mAM forms a complex with ESET, stimulating its methyltransferase activity, specifically the conversion of di-methyl to tri-methyl histone H3 Lys9 (2). MBD1 recruits ESET to the CAF-1 complex to facilitate methylation of histone H3 Lys9 during replication-coupled chromatin assembly in S phase (5). DNMT3A recruits ESET to silenced promoters in cancer cells (7). ESET may play a role in the pathogenesis of Huntington's disease, since levels of ESET protein and tri-methyl histone H3 Lys9 are both increased in diseased brains (8).
-
Yang, L. et al. (2002) Oncogene 21, 148-152.
-
Wang, H. et al. (2003) Mol. Cell 12, 475-487.
-
Schultz, D.C. et al. (2002) Genes Dev. 16, 919-932.
-
Yang, L. et al. (2003) Biochem. J. 369, 651-657.
-
Sarraf, S.A. and Stancheva, I. (2004) Mol. Cell 15, 595-605.
-
Ichimura, T. et al. (2005) J. Biol. Chem. 280, 13928-13935.
-
Li, H. et al. (2006) J. Biol. Chem. 281, 19489-19500.
-
Ryu, H. et al. (2006) Proc. Natl. Acad. Sci. USA 103, 19176-19181.
PhosphoSitePlus®：
SETDB1