Cell Cycle / Checkpoint Control
RPA70 (4D9) Rat mAb
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| CSTコード |
包装 |
希望納入価格 (円) |
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| #2198S | 100 μL | 46,000 | |
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RPA70抗体製品一覧
2198 の推奨プロトコール
最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。
注：各製品に最適化されたプロトコールをリンクしています。
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2198:
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Immunofluorescence
Immunoprecipitation
Western Blotting
| 用途 (希釈倍率) | |
| ウェスタンブロッティング (1:1,000)、免疫沈降 (1:50)、免疫蛍光細胞染色 (IF-IC) (1:100) |
| 特異性・感度 | |
| 内在性レベルのRPA70 タンパク質を検出します。 |
| 使用抗原 | |
| ヒトのRPA70 タンパク質のC末端領域 (組換えMBP 融合タンパク質) |
Western Blotting
Western blot analysis of extracts from various cell lines, using RPA70 (4D9) Rat mAb.
IF-IC
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or UV-treated (right), using RPA70 (4D9) Rat mAb (green) showing translocation to distinct nuclear foci after UV damage. Actin filaments have been labeled with Alexa Fluor® 555 phalloidin. Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
RPA70 (HSSB, REPA1, RF-A, RP-A, p70) is a component of a heterotrimeric complex, composed of 70, 32/30 and 14 kDa subunits, collectively known as RPA. RPA is a single stranded DNA binding protein, whose DNA binding activity is believed to reside entirely in the 70 kDa subunit. The complex is required for almost all aspects of cellular DNA metabolism such as DNA replication (1-3), recombination, cell cycle and DNA damage checkpoints, and all major types of DNA repair including nucleotide excision, base excision, mismatch and double-strand break repairs (4-7). In response to genotoxic stress in eukaryotic cells, RPA has been shown to associate with the Rad9/Rad1/Hus1 (9-1-1) checkpoint complex (8). RPA is hyperphosphorylated upon DNA damage or replication stress by checkpoint kinases including ataxia telangiectasia mutated (ATM), ATM and Rad3-related (ATR), and DNA-dependent protein kinase (DNA-PK) (9-11). Hyperphosphorylation may alter RPA-DNA and RPA-protein interactions. In addition to the checkpoint partners, RPA interacts with a wide variety of protein partners, including proteins required for normal replication such as RCF, PCNA and Pol α, and also proteins involved in SV40 replication, such as DNA polymerase I and SV40 large T antigen (10,12).
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Liu, V.F. and Weaver, D.T. (1993) Mol. Cell Biol. 13, 7222-7231.
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Wobbe, C.R. et al. (1987) Proc. Natl. Acad. Sci. USA 84, 1834-1838.
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Fairman, M.P. and Stillman, B. (1988) EMBO J. 7, 1211-1218.
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Wold, M.S. and Kelly, T. (1988) Proc. Natl. Acad. Sci. USA 85, 2523-2527.
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Zhou, B.B. and Elledge, S.J. (2000) Nature 408, 433-439.
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Kastan, M.B. and Bartek, J. (2004) Nature 432, 316-323.
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Sancar, A. et al. (2004) Annu. Rev. Biochem. 73, 39-85.
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Guo, S. et al. (2006) J Biol Chem 281, 21607-16.
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Wu, X. et al. (2005) Oncogene 24, 4728-4735.
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Binz, S.K. et al. DNA Repair (Amst) 3, 1015-1024.
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Nuss, J.E. et al. (2005) Biochemistry 44, 8428-8437.
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Yuzhakov, A. et al. (1999) EMBO J. 18, 6189-6199.
PhosphoSitePlus®：
RPA1