Translational Control

Translational Control

S6 Ribosomal Protein (5G10) Rabbit mAb

CSTコード	包装	希望納入価格（円）	国内在庫 2010年3月11日15時15分現在	ご登録代理店情報カスタマー情報にご登録いただいた代理店を表示しています。ご登録代理店の変更はこちら。
#2217	100μL	46,000		ログインすると国内在庫状況がご確認いただけます。
#2217L	300 μL	111,000

用途（希釈倍率）
ウエスタンブロッティング(1:1,000)、免疫組織染色（パラフィン）（1:100）、免疫蛍光細胞染色（IF-IC）（1:100）

種交差性
ヒト、マウス、ラット、サル、

特異性・感度
リン酸化に関わらず、内在性レベルのS6 Ribosomal Protein を検出します。

検出タンパク質の分子量
32 kDa

使用抗原
ヒトS6 Ribosomal Protein 由来の配列（合成ペプチド）

抗体の由来
ウサギ

貯法
-20℃

Western Blotting

Western blot analysis of extracts from HeLa, NIH/3T3, PC12 and COS cells using S6 Ribosomal Protein (5G10) Rabbit mAb.

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using S6 Ribosomal Protein (5G10) Rabbit mAb.

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using S6 Ribosomal Protein (5G10) Rabbit mAb.

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma showing cytoplasmic localization using S6 Ribosomal Protein (5G10) Rabbit mAb.

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human Non-Hodgkin's lymphoma, using S6 Ribosomal Protein (5G10) Rabbit mAb.

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using S6 Ribosomal Protein (5G10) Rabbit mAb.

IF-IC

Confocal immunofluorescent images of HeLa cells labeled with S6 Ribosomal Protein (5G10) Rabbit mAb (red, left) compared to an isotype control (right). Actin filaments have been labeled with fluorescein phalloidin. Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).

バックグラウンド

One way that growth factors and mitogens effectively promote sustained cell growth and proliferation is by upregulating mRNA translation (1,2). Growth factors and mitogens induce the activation of p70 S6 kinase and the subsequent phosphorylation of the S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation of mRNA transcripts that contain an oligopyrimidine tract in their 5' untranslated regions (2). These particular mRNA transcripts (5'TOP) encode proteins involved in cell cycle progression as well as ribosomal proteins and elongation factors necessary for translation (2,3). Important S6 ribosomal protein phosphorylation sites include several residues (Ser235, Ser236, Ser240 and Ser244) located within a small, carboxy-terminal region of the S6 protein (4,5).

使用文献
Guertin, D.A. et al. (2009) Cancer Cell 15, 148-59. This article references the use of S6 Ribosomal Protein (5G10) Rabbit mAb in the following applications: :Western Blotting: Dufner, A. et al. (1999) Ribosomal S6 kinase signaling and the control of translation. Exp Cell Res. 1999 Nov 25;253(1):100-9. This article references the use of S6 Ribosomal Protein (5G10) Rabbit mAb Peterson, RT, et al. (1998) Translation control: connecting mitogens and the ribosome. Curr Biol. 1998 Mar 26;8(7):R248-50. This article references the use of S6 Ribosomal Protein (5G10) Rabbit mAb Jefferies, HB, et al. (1997) Rapamycin suppresses 5'TOP mRNA translation through inhibition of p70s6k. EMBO J. 1997 Jun 16;16(12):3693-704. This article references the use of S6 Ribosomal Protein (5G10) Rabbit mAb Ferrari, S. et al. (1991) Mitogen-activated 70K S6 kinase. Identification of in vitro 40 S ribosomal S6 phosphorylation sites. J Biol Chem. 1991 Nov 25;266(33):22770-5. This article references the use of S6 Ribosomal Protein (5G10) Rabbit mAb Flotow, H. et al. (1992) Substrate recognition determinants of the mitogen-activated 70K S6 kinase from rat liver. J Biol Chem. 1992 Feb 15;267(5):3074-8. This article references the use of S6 Ribosomal Protein (5G10) Rabbit mAb

使用文献

Guertin, D.A. et al. (2009) Cancer Cell 15, 148-59. This article references the use of S6 Ribosomal Protein (5G10) Rabbit mAb in the following applications: :Western Blotting:
Dufner, A. et al. (1999) Ribosomal S6 kinase signaling and the control of translation.
Exp Cell Res. 1999 Nov 25;253(1):100-9. This article references the use of S6 Ribosomal Protein (5G10) Rabbit mAb
Peterson, RT, et al. (1998) Translation control: connecting mitogens and the ribosome. Curr Biol. 1998 Mar 26;8(7):R248-50.
This article references the use of S6 Ribosomal Protein (5G10) Rabbit mAb
Jefferies, HB, et al. (1997) Rapamycin suppresses 5'TOP mRNA translation through inhibition of p70s6k. EMBO J. 1997 Jun 16;16(12):3693-704. This article references the use of S6 Ribosomal Protein (5G10) Rabbit mAb
Ferrari, S. et al. (1991) Mitogen-activated 70K S6 kinase. Identification of in vitro 40 S ribosomal S6 phosphorylation sites. J Biol Chem. 1991 Nov 25;266(33):22770-5. This article references the use of S6 Ribosomal Protein (5G10) Rabbit mAb
Flotow, H. et al. (1992) Substrate recognition determinants of the mitogen-activated 70K S6 kinase from rat liver. J Biol Chem. 1992 Feb 15;267(5):3074-8. This article references the use of S6 Ribosomal Protein (5G10) Rabbit mAb

関連製品
2211 Phospho-S6 Ribosomal Protein (Ser235/236) Antibody
2215 Phospho-S6 Ribosomal Protein (Ser240/244) Antibody
2317 S6 Ribosomal Protein (54D2) Mouse mAb
4858 Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP™ Rabbit mAb
7003 20X LumiGLO Reagent and 20X Peroxide
7071 Phototope-HRP Western Blot Detection System (with Anti-rabbit IgG, HRP-linked Antibody)
7074 Anti-rabbit IgG, HRP-linked Antibody
7720 Prestained Protein Marker, Broad Range (Premixed Format)
7727 Biotinylated Protein Ladder Detection Pack
8101 SignalSlide Phospho-Akt (Ser473) IHC Controls
8112 SignalStain® Antibody Diluent
9203 p70 S6 Kinase Control Cell Extracts

本製品は試験研究用です。

S6 Ribosomal Protein (5G10) Rabbit mAb