Angiogenesis
| CSTコード |
包装 |
希望納入価格（円） |
国内在庫  |
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| #2227S | 100 μL | 57,000 | |
|
| #2227L | 300 μL | 137,000 | |
PDGFR-b抗体製品一覧
推奨プロトコール
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2227:
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Western Blotting
| 用途（希釈倍率） | |
| ウェスタンブロッティング（1:1,000） |
| 特異性・感度 | |
| 内在性レベルのTyr1021 がリン酸化されたPDGF Receptor βタンパク質を検出します。また、活性化PDGF Receptor ファミリータンパク質およびEGFR 等の活性化チロシンキナーゼとも交差する可能性があります。 |
| 使用抗原 | |
| ヒトのPDGF Receptor βタンパク質のTyr1021 周辺領域（合成ペプチド） |
Western Blotting
Western blot analysis of extracts of NIH/3T3 cells untreated or treated with PDGF-BB, using Phospho-PDGF Receptor β (Tyr1021) (6F10) Rabbit mAb (upper), or PDGF Receptor β (2B3) Mouse mAb (#3175) (lower).
The proteins of the platelet derived growth factor (PDGF) family exist as several disulphide-bonded, dimeric isoforms (PDGF AA, PDGF AB, PDGF BB, PDGF CC and PDGF DD) that bind in a specific pattern to two closely related receptor tyrosine kinases, PDGF receptor α (PDGFRα) and PDGF receptor β (PDGFRβ). PDGFRα and PDGFRβ share 75% to 85% sequence homology between their two intracellular kinase domains while the kinase insert and carboxy-terminal tail regions display a lower level (27% to 28%) of homology (1). PDGF Receptor α homodimers bind all PDGF isoforms except those containing PDGF D. PDGF Receptor β homodimers bind PDGF BB and DD isoforms, as well as the PDGF AB heterodimer. The heteromeric PDGFα/β receptor binds PDGF B, C, and D homodimers as well as the PDGF AB heterodimer (2). PDGFRα and PDGFRβ can each form heterodimers with EGFR, which is also activated by PDGF (3). Various cells differ in the total number of receptors present and in the receptor subunit composition, which may account for responsive differences among cell types to PDGF binding (4). Ligand binding induces receptor dimerization and autophosphorylation, followed by binding and activation of cytoplasmic SH2 domain-containing signal transduction molecules such as Grb2, Src, GAP, PI3 kinase, PLCγ and Nck. A number of different signaling pathways are initiated by activated PDGF receptors and lead to control of cell growth, actin reorganization, migration and differentiation (5). Tyr751 in the kinase-insert region of PDGFRβ is the docking site for PI3 kinase (6). Phosphorylated pentapeptides derived from Tyr751 of PDGFRβ (pTyr751-Val-Pro-Met-Leu) inhibit the association of the carboxy-terminal SH2 domain of the p85 subunit of PI3 kinase with PDGFRβ (7). Tyr740 is also required for PDGFRβ mediated PI3 kinase activation (8).
PDGF-stimulated PLCγ signaling is dependent on autophosphorylation of the PDGF β receptor at Tyr1009 and Tyr1021 (8). It was also shown that both Tyr1009 and Tyr1021 alone and in cooperation mediate PDGF-BB triggered calcium signalling (9).
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