Tyrosine Kinases / Adaptors
Phospho-EGF Receptor (Tyr845) Antibody
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| CSTコード |
包装 |
希望納入価格 (円) |
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| #2231S | 100 μL | 57,000 | |
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| #2231L | 300 μL | 137,000 | |
EGFR抗体製品一覧
2231 の推奨プロトコール
最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。
注：各製品に最適化されたプロトコールをリンクしています。
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2231:
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Western Blotting
| 用途（希釈倍率） | |
| ウエスタンブロッティング(1:1,000)、免疫沈降(1:100)、免疫組織染色(1:400)、免疫細胞染色(1:400)、フローサイトメトリー(1:25) |
| 特異性・感度 | |
| 内在性レベルのTyr845がリン酸化されたEGF Receptor を検出します。他の活性化EGF Receptor ファミリーも検出する可能性があります。 |
| 使用抗原 | |
| ヒトのEGF Receptor のTyr845 周辺領域（合成ペプチド） |
Western Blotting
Western blot analysis of extracts from A431 cells, untreated or EGF-stimulated (100 ng/ml), using Phospho-EGF Receptor (Tyr845) Antibody (upper) or EGF Receptor (C74B9) Rabbit mAb #2646 (lower).
The epidermal growth factor (EGF) receptor is a transmembrane tyrosine kinase that belongs to the HER/ErbB protein family. Ligand binding results in receptor dimerization, autophosphorylation, activation of downstream signaling, internalization, and lysosomal degradation (1,2). Phosphorylation of EGF receptor (EGFR) at Tyr845 in the kinase domain is implicated in stabilizing the activation loop, maintaining the active state enzyme, and providing a binding surface for substrate proteins (3,4). c-Src is involved in phosphorylation of EGFR at Tyr845 (5). The SH2 domain of PLCγ binds at phospho-Tyr992, resulting in activation of PLCγ-mediated downstream signaling (6). Phosphorylation of EGFR at Tyr1045 creates a major docking site for the adaptor protein c-Cbl, leading to receptor ubiquitination and degradation following EGFR activation (7,8). The GRB2 adaptor protein binds activated EGFR at phospho-Tyr1068 (9). A pair of phosphorylated EGFR residues (Tyr1148 and Tyr1173) provide a docking site for the Shc scaffold protein, with both sites involved in MAP kinase signaling activation (2). Phosphorylation of EGFR at specific serine and threonine residues attenuates EGFR kinase activity. EGFR carboxy-terminal residues Ser1046 and Ser1047 are phosphorylated by CaM kinase II; mutation of either of these serines results in upregulated EGFR tyrosine autophosphorylation (10).
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Hackel, P.O. et al. (1999) Curr Opin Cell Biol 11, 184-9.
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Zwick, E. et al. (1999) Trends Pharmacol Sci 20, 408-12.
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Cooper, J.A. and Howell, B. (1993) Cell 73, 1051-4.
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Hubbard, S.R. et al. (1994) Nature 372, 746-54.
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Biscardi, J.S. et al. (1999) J Biol Chem 274, 8335-43.
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Emlet, D.R. et al. (1997) J Biol Chem 272, 4079-86.
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Levkowitz, G. et al. (1999) Mol Cell 4, 1029-40.
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Ettenberg, S.A. et al. (1999) Oncogene 18, 1855-66.
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Rojas, M. et al. (1996) J Biol Chem 271, 27456-61.
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Feinmesser, R.L. et al. (1999) J Biol Chem 274, 16168-73.
- Wu, W. et al. (2002) Src-dependent phosphorylation of the epidermal growth factor receptor on tyrosine 845 is required for zinc-induced Ras activation. J. Biol. Chem. 277, 24252-24257. Applications: Western Blotting
- Iordanov, M. S. et al. (2002) The UV (ribotoxic) stress response of human keratinocytes involves the unexpected uncoupling of the Ras-extracellular signal-regulated kinase signaling cascade from the activated epidermal growth factor receptor. Mol. Cell. Biol. 22, 5380-5394. Applications: Western Blotting
- Zhang, B. et al. (2002) E5 Protein of Human Papillomavirus Type 16 Protects Human Foreskin Keratinocytes from UV B-Irradiation-Induced Apoptosis. J. Virol. 76, 220-231. Applications: Western Blotting
- Ravid, T. et al. (2002) Epidermal Growth Factor Receptor Activation under Oxidative Stress Fails to Promote c-Cbl Mediated Down-regulation. J. Biol. Chem. 277, 31214-31219. Applications: Western Blotting
- Nishimura, M. et al. (2009) Mol Cell Biol 29, 5529-39. Applications: Western Blotting
PhosphoSitePlus®：
EGFR