MAP Kinase Signaling
- MAP Kinase Signaling
PhosphoSitePlus®：
FosB
- 推奨プロトコール
FosB (5G4) Rabbit mAb |
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| CSTコード | 包装 | 希望納入価格 (円) |
国内在庫  2012年2月9日15時20分 現在 |
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| #2251S | 100 μL | 46,000 | ログインすると国内在庫状況がご確認いただけます。
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2251 の推奨プロトコール 最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。 注：各製品に最適化されたプロトコールをリンクしています。 |
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下記ステップについては、データシートの右側もあわせてご参照ください。
IHC-P: 抗体希釈液 / 抗原賦活化
| 用途 (希釈倍率) | |
|---|---|
| ウェスタンブロッティング (1:1,000)、免疫沈降 (1:50)、免疫組織染色 (パラフィン) (1:25)、免疫蛍光細胞染色 (IF‐IC) (1:200)、フローサイトメトリー (1:100) |
| 種交差性 | |
|---|---|
| ヒト、マウス、ラット |
| 特異性・感度 | |
|---|---|
| 内在性レベルのFosB タンパク質 (FosB とFosB2 Isoform 両方) を検出します。c-Fos、FRA1、FRA2 等の他のFos タンパク質とは交差しません。 |
| 検出タンパク質の分子量 | |
|---|---|
| 38 kDa (FosB2)、48 kDa (FosB) |
| 使用抗原 | |
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| ヒトのFosB タンパク質の配列 (合成ペプチド) |
| 抗体の由来 | |
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| ウサギ |
| 貯法 | |
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| -20℃ |
| 社内データ |
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Western Blotting
Western blot analysis of extracts from HeLa cells serum-starved overnight and TPA-stimulated for 4 hours, or NIH/3T3 cells and C6 cells serum-starved overnight and serum-stimulated for 4 hours, using FosB (5G4) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using FosB (5G4) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using FosB (5G4) Rabbit mAb in the presence of control peptide (left) or FosB Blocking Peptide #1042 (right).
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded HeLa cells control (left) or PMA-treated (right), using FosB (5G4) Rabbit mAb.
Flow Cytometry
Flow cytometric analysis of HeLa cells, untreated (blue) or TPA treated (green), using FosB (5G4) Rabbit mAb compared to a nonspecific negative control antibody (red).
IF-IC
Confocal immunofluorescent analysis of HeLa cells either serum-starved (left) or TPA-treated (right) and labeled with FosB (5G4) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red).
| バックグラウンド |
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The Fos family of nuclear oncogenes includes c-Fos, FosB, Fos-related antigen 1 (FRA1), and Fos-related antigen 2 (FRA2) (1). While most Fos proteins exist as a single isoform, the FosB protein exists as two isoforms: full-length FosB and a shorter form, FosB2 (Delta FosB), that lacks the carboxy-terminal 101 amino acids (1-3). The expression of Fos proteins is rapidly and transiently induced by a variety of extracellular stimuli including growth factors, cytokines, neurotransmitters, polypeptide hormones, and stress. Fos proteins dimerize with Jun proteins (c-Jun, JunB, and JunD) to form Activator Protein-1 (AP-1), a transcription factor that binds to TRE/AP-1 elements and activates transcription. Fos and Jun proteins contain the leucine-zipper motif that mediates dimerization and an adjacent basic domain that binds to DNA. The various Fos/Jun heterodimers differ in their ability to transactivate AP-1 dependent genes. In addition to increased expression, phosphorylation of Fos proteins by Erk kinases in response to extracellular stimuli may further increase transcriptional activity (4-6). Phosphorylation of c-Fos at Ser32 and Thr232 by Erk5 increases protein stability and nuclear localization (5). Phosphorylation of FRA1 at Ser252 and Ser265 by Erk1/2 increases protein stability and leads to overexpression of FRA1 in cancer cells (6). Following growth factor stimulation, expression of FosB and c-Fos in quiescent fibroblasts is immediate, but very short-lived, with protein levels dissipating after several hours (7). FRA1 and FRA2 expression persists longer and appreciable levels can be detected in asynchronously growing cells (8). Deregulated expression of c-Fos, FosB, or FRA2 can result in neoplastic cellular transformation; however, Delta FosB lacks the ability to transform cells (2,3).
- Tulchinsky, E. (2000) Histol. Histopathol. 15, 921-928.
- Dobrzanski, P. et al. (1991) Mol. Cell. Biol. 11, 5470-5478.
- Nakabeppu, Y. and Nathans, D. (1991) Cell 64, 751-759.
- Rosenberger, S.F. et al. (1999) J. Biol. Chem. 274, 1124-1130.
- Sasaki, T. et al. (2006) Mol. Cell 24, 63-75.
- Basbous, J. et al. (2007) Mol. Cell. Biol. 27, 3936-3950.
- Kovary, K. and Bravo, R. (1991) Mol. Cell. Biol. 11, 2451-2459.
- Kovary, K. and Bravo, R. (1992) Mol. Cell. Biol. 12, 5015-5023.
| 使用例 | |
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| 製品をご使用いただいて研究を発表されましたら、ぜひお知らせください。 |
本製品は試験研究用です。