Phosphatases
PP2A A Subunit (6G3) Rat mAb
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| CSTコード |
包装 |
希望納入価格 (円) |
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| #2260S | 100 μL | 46,000 | |
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PP2A抗体製品一覧
2260 の推奨プロトコール
最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。
注：各製品に最適化されたプロトコールをリンクしています。
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2260:
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Western Blotting
| 用途（希釈倍率） | |
| ウェスタンブロッティング（1:1,000） |
| 種交差性 | |
| ヒト、マウス、ラット、サル、（ウシ、アフリカツメガエル） |
| 特異性・感度 | |
| 内在性レベルのPP2A A Subunit のα アイソフォームを検出します。また、β アイソフォームも検出する可能性があります。他のPP2A Subunit とは交差しません。 |
Western Blotting
Western blot analysis of extracts from HeLa, NIH/3T3, C6 and COS cells, using PP2A A Subunit (6G3) Rat mAb.
Protein phosphatase type 2A (PP2A) is an essential protein serine/threonine phosphatase that is conserved in all eukaryotes. PP2A is a key enzyme within various signal transduction pathways as it regulates fundamental cellular activities such as DNA replication, transcription, translation, metabolism, cell cycle progression, cell division, apoptosis and development (1-3). The core enzyme consists of catalytic C and regulatory A (or PR65) subunits, with each subunit represented by α and β isoforms (1). Additional regulatory subunits belong to four different families of unrelated proteins. Both the B (or PR55) and B' regulatory protein families contain α, β, γ and δ isoforms, with the B' family also including an ε protein. B'' family proteins include PR72, PR130, PR59 and PR48 isoforms, while striatin (PR110) and SG2NA (PR93) are both members of the B''' regulatory protein family. These B subunits competitively bind to a shared binding site on the core A subunit (1). This variable array of holoenzyme components, particularly regulatory B subunits, allows PP2A to act in a diverse set of functions. PP2A function is regulated by expression, localization, holoenzyme composition and post-translational modification. Phosphorylation of PP2A at Tyr307 by Src occurs in response to EGF or insulin and results in a substantial reduction of PP2A activity (4). Reversible methylation on the carboxyl group of Leu309 of PP2A has been observed (5,6). Methylation alters the conformation of PP2A, as well as its localization and association with B regulatory subunits (6-8).
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Janssens, V. and Goris, J. (2001) Biochem. J. 353, 417-439.
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Zolnierowicz, S. (2000) Biochem. Pharmacol. 60, 1225-1235.
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Milward, T.A. et al. (1999) Trends Biochem. Sci. 24, 186-191.
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Chen, J. et al. (1992) Science 257, 1261-1264.
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Turowski, P. et al. (1995) J. Cell. Biol. 129, 397-410.
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Lee, J. et al. (1996) Proc. Natl. Acad. Sci. USA 93, 6043-6047.
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Tolstykh, T. et al. (2000) EMBO J. 19, 5682-5691.
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Yu, X.X. et al. (2001) Mol. Biol. Cell 12, 185-199.
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Kremmer, E. et al. (1997) Mol Cell Biol 17, 1692-701.
PhosphoSitePlus®：
PPP2R1A