Lymphocyte Signaling
- Lymphocyte Signaling
PhosphoSitePlus®：
PU.1
- 推奨プロトコール
PU.1 Antibody |
イイネ！(0) | 
|
| CSTコード | 包装 | 希望納入価格 (円) |
国内在庫  2012年2月9日11時30分 現在 |
|
|---|---|---|---|---|
| #2266S | 100 μL | 46,000 | ログインすると国内在庫状況がご確認いただけます。
|
|
2266 の推奨プロトコール 最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。 注：各製品に最適化されたプロトコールをリンクしています。 |
|---|
- 2266:
- ChIP Agarose ChIP Magnetic Flow IHC / Paraffin Immunofluorescence Immunoprecipitation Western Blotting
下記ステップについては、データシートの右側もあわせてご参照ください。
IHC-P: 抗体希釈液 / 抗原賦活化
| 用途（希釈倍率） | |
|---|---|
| ウェスタンブロッティング（1:1,000）、免疫沈降（1:100）、免疫組織染色（パラフィン）（1:200）、免疫細胞染色（IF-IC）（1:100）、フローサイトメトリー（1:50）、ChIP（1:25） |
| 種交差性 | |
|---|---|
| ヒト、マウス、（サル） |
| 特異性・感度 | |
|---|---|
| 内在性レベルのPU.1 タンパク質を検出します。他のEts ファミリータンパク質とは交差しません。 |
| 検出タンパク質の分子量 | |
|---|---|
| 42 kDa |
| 使用抗原 | |
|---|---|
| ヒトのPU.1 タンパク質のN末端領域のアミノ酸（合成ペプチド） |
| 抗体の由来 | |
|---|---|
| ウサギ |
| 貯法 | |
|---|---|
| -20℃ |
| ※括弧付きの動物種は配列が100%相同であるため反応すると推定されます。 |
| 社内データ |
|---|
Western Blotting
Western blot analysis of extracts from RAW, p388D1, WEHI-3 and THP1 cells using #2266.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing nuclear localization in lymphocytes, using PU.1 Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma (infiltrating cells), using PU.1 Antibody in the presence of control peptide (left) or PU.1 Blocking Peptide #1036 (right).
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human tonsil, showing nuclear localization, using PU.1 Antibody.
Flow Cytometry
Flow cytometric analysis of RAW cells, using PU.1 antibody (blue) compared to a nonspecific negative control antibody (red).
IF-IC
DAPI staining (left) and immunofluorescent staining (right) of paraformaldehyde-fixed RAW cells using #2266.
Chromatin IP
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 U-937 cells and either 20 μl of PU.1 Antibody or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human CD11b promoter primers, human CD18 intron 1 primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
| バックグラウンド |
|---|
PU.1 is a member of the Ets family of transcription factors and activates target genes through the purine-rich PU-box (1). PU.1 plays a pivotal role in the differentiation of myeloid cells and lymphocytes and is expressed in several hematopoietic cells including B lymphocytes, macrophages, neutrophils, mast cells, early erythroid cells and megakaryocytes (1,2). The concentration of PU.1 is critical for both the determination of hematopoietic cell lineage and the regulation of differentiation versus stem cell proliferation (3,4). In addition, PU.1 activity is influenced by phosphorylation and interactions with other hematopoietic transcription factors. Phosphorylation of PU.1 at Ser146 by CK2 promotes binding to IRF4 and synergistic activation through the immunoglobulin κ 3' enhancer (5). Treatment of pro-B cells with IL-3 leads to phosphorylation of PU.1 at Ser140, resulting in increased PU.1 activity and activation of the anti-apoptotic gene MCL-1 (6). GATA1 binding blocks PU.1 activity during erythroid cell development (7). Overexpression of PU.1 resulting from proviral insertion during Friend virus infection can induce erythroleukemia, while reduced expression has been associated with acute myeloid leukemia (8).
- Lloberas, J. et al. (1999) Immunol. Today 20, 184-189.
- Klemsz, M.J. et al. (1990) Cell 61, 113-124.
- Dahl, R. and Simon, M.C. (2003) Blood Cells Mol. Dis. 31, 229-233.
- DeKoter, R.P. and Singh, H. (2000) Science 288, 1439-1441.
- Pongubala, J.M. et al. (1993) Science 259, 1622-1625.
- Wang, J.M. et al. (2003) Mol. Cell Biol. 23, 1896-1909.
- Zhang, P. et al. (1999) Proc. Natl. Acad. Sci. USA 96, 8705-8710.
- Moreau-Gachelin, F. et al. (1998) Nature 331, 277-280.
| 使用例 | |
|---|---|
| 製品をご使用いただいて研究を発表されましたら、ぜひお知らせください。 |
本製品は試験研究用です。