DNA Damage
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| CSTコード | 包装 | 希望納入価格（円） |
国内在庫  2010年7月30日15時15分 現在 |
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| #2267 | 100 μL | 46,000 | ログインすると国内在庫状況がご確認いただけます。
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| 用途（希釈倍率） | |
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| ウエスタンブロッティング(1:1,000)、免疫沈降（1:50）、免疫細胞染色（IF）（1:25）、フローサイトメトリー（1:25） | |
| 種交差性 | |
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| ヒト、ラット、サル | |
| 特異性・感度 | |
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| 内在性レベルのRPA70 サブユニットを検出します。 | |
| 検出タンパク質の分子量 | |
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| 70 kDa | |
| 使用抗原 | |
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| ヒトのRPA70 のN 末端領域（合成ペプチド） | |
| 抗体の由来 | |
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| ウサギ | |
| 貯法 | |
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| -20℃ | |
| 社内データ |
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Western Blotting
Western blot analysis of extracts from A431 and K562 cells, using RPA70 Antibody.
Flow Cytometry
Flow cytometric analysis of Jurkat cells, using RPA70 antibody (blue) compared to a nonspecific negative control antibody (red).
IF-IC
Confocal immunofluorescent images of HeLa cells, untreated (left) or UV-treated (right), labeled with RPA70 Antibody (green) showing translocation to distinct nuclear foci after UV damage. Actin filaments have been labeled with Alexa Fluor® 555 phalloidin. Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).
| バックグラウンド |
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RPA70 (HSSB, REPA1, RF-A, RP-A, p70) is a component of a heterotrimeric complex, composed of 70, 32/30 and 14 kDa subunits, collectively known as RPA. RPA is a single stranded DNA binding protein, whose DNA binding activity is believed to reside entirely in the 70 kDa subunit. The complex is required for almost all aspects of cellular DNA metabolism such as DNA replication (1-3), recombination, cell cycle and DNA damage checkpoints, and all major types of DNA repair including nucleotide excision, base excision, mismatch and double-strand break repairs (4-7). In response to genotoxic stress in eukaryotic cells, RPA has been shown to associate with the Rad9/Rad1/Hus1 (9-1-1) checkpoint complex (8). RPA is hyperphosphorylated upon DNA damage or replication stress by checkpoint kinases including ataxia telangiectasia mutated (ATM), ATM and Rad3-related (ATR), and DNA-dependent protein kinase (DNA-PK) (9-11). Hyperphosphorylation may alter RPA-DNA and RPA-protein interactions. In addition to the checkpoint partners, RPA interacts with a wide variety of protein partners, including proteins required for normal replication such as RCF, PCNA and Pol α, and also proteins involved in SV40 replication, such as DNA polymerase I and SV40 large T antigen (10,12).
- Liu, V.F. and Weaver, D.T. (1993) Mol. Cell Biol. 13, 7222-7231.
- Wobbe, C.R. et al. (1987) Proc. Natl. Acad. Sci. USA 84, 1834-1838.
- Fairman, M.P. and Stillman, B. (1988) EMBO J. 7, 1211-1218.
- Wold, M.S. and Kelly, T. (1988) Proc. Natl. Acad. Sci. USA 85, 2523-2527.
- Zhou, B.B. and Elledge, S.J. (2000) Nature 408, 433-439.
- Kastan, M.B. and Bartek, J. (2004) Nature 432, 316-323.
- Sancar, A. et al. (2004) Annu. Rev. Biochem. 73, 39-85.
- Guo, S. et al. (2006) J Biol Chem 281, 21607-16.
- Wu, X. et al. (2005) Oncogene 24, 4728-4735.
- Binz, S.K. et al. DNA Repair (Amst) 3, 1015-1024.
- Nuss, J.E. et al. (2005) Biochemistry 44, 8428-8437.
- Yuzhakov, A. et al. (1999) EMBO J. 18, 6189-6199.
| 使用例 | |
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| 製品をご使用いただいて研究を発表されましたら、ぜひお知らせください。 |
本製品は試験研究用です。
