MAP Kinase Signaling
JNK3 (55A8) Rabbit mAb
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| CSTコード |
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| #2305S | 100 μL | 46,000 | |
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JNK3抗体製品一覧
2305 の推奨プロトコール
最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。
注：各製品に最適化されたプロトコールをリンクしています。
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2305:
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Western Blotting
| 用途（希釈倍率） | |
| ウェスタンブロッティング（1:1,000） |
| 特異性・感度 | |
| 内在性レベルのJNK3 タンパク質を検出します。 |
| 使用抗原 | |
| ヒトのJNK3 タンパク質（合成ペプチド） |
Western Blotting
Western blot analysis of recombinant His-tagged JNK kinases (left), or brain lysates from wild type (WT) and specific JNK knockout (-/-) mice (right), using JNK3 (55A8) Rabbit mAb (Image provided by Drs Gerardo Morfini, YiMei You and Scott Brady, University of Illinois at Chicago).
Western Blotting
Western blot analysis of extracts from human cerebellum, mouse brain and rat brain, using JNK3 (55A8) Rabbit mAb.
The stress-activated protein kinase/Jun-amino-terminal kinase SAPK/JNK is potently and preferentially activated by a variety of environmental stresses including UV and gamma radiation, ceramides, inflammatory cytokines, and in some instances, by growth factors and GPCR agonists (1-6). As with the other MAPKs, the core signaling unit is composed of a MAPKKK, typically MEKK1-MEKK4, or by one of the mixed lineage kinases (MLKs), which phosphorylate and activate MKK4/7. Upon activation, MKKs phosphorylate and activate the SAPK/JNK kinase (2). Stress signals are delivered to this cascade by small GTPases of the Rho family (Rac, Rho, cdc42) (3). Both Rac1 and cdc42 mediate the stimulation of MEKKs and MLKs (3). Alternatively, MKK4/7 can be activated in a GTPase-independent mechanism via stimulation of a germinal center kinase (GCK) family member (4). There are three SAPK/JNK genes each of which undergoes alternative splicing resulting in numerous isoforms (3). SAPK/JNK, when active as a dimer, can translocate to the nucleus and regulate transcription through its effects on c-Jun, ATF-2, and other transcription factors (3,5).
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Davis, R.J. (1999) Biochem Soc Symp 64, 1-12.
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Ichijo, H. (1999) Oncogene 18, 6087-93.
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Kyriakis, J.M. and Avruch, J. (2001) Physiol Rev 81, 807-69.
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Kyriakis, J.M. (1999) J Biol Chem 274, 5259-62.
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Leppä, S. and Bohmann, D. (1999) Oncogene 18, 6158-62.
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Whitmarsh, A.J. and Davis, R.J. (1998) Trends Biochem Sci 23, 481-5.
PhosphoSitePlus®：
JNK3