Adhesion
Rap1B (36E1) Rabbit mAb
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| CSTコード |
包装 |
希望納入価格 (円) |
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| #2326S | 100 μL | 46,000 | |
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Rap1B抗体製品一覧
2326 の推奨プロトコール
最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。
注：各製品に最適化されたプロトコールをリンクしています。
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2326:
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Western Blotting
| 用途 (希釈倍率) | |
| ウェスタンブロッティング (1:1,000) |
| 特異性・感度 | |
| 内在性レベルのRap1B タンパク質を検出します。Rap1A タンパク質とは交差しません。 |
| 使用抗原 | |
| ヒトのRap1B タンパク質のC末端近傍領域 (合成ペプチド) |
Western Blotting
Western blot analysis of cell extracts from various cell lines using Rap1B (36E1) Rabbit mAb.
Western Blotting
Western blot analysis of HeLa cell lysates using Rap1B (36E1) Rabbit mAb without pre-absorption (A), or pre-incubated with Rab1A (B) and Rap1B (C) carboxy-terminal peptides respectively. The result showed that only Rap1B peptide specifically blocks the antibody binding in Western blot.
Rap1 and Rap2 belong to the Ras subfamily of small GTPases and are activated by a wide variety of stimuli through integrins, receptor tyrosine kinases (RTKs), G-protein coupled receptors (GPCR), death domain associated receptors (DD-R) and ion channels (1,2). Like other small GTPases, Rap activity is stimulated by guanine nucleotide exchange factors (GEF) and inactivated by GTPase activating proteins (GAP). A wide variety of Rap GEFs have been identified: C3G connects Rap1 with RTKs through adaptor proteins such as Crk, Epacs (or cAMP-GEFs) transmit signals from cAMP, and CD-GEFs (or CalDAG-GEFs) convey signals from either or both Ca2+ and DAG (1). Rap1 primarily regulates multiple integrin-dependent processes such as morphogenesis, cell-cell adhesion, hematopoiesis, leukocyte migration and tumor invasion (1,2). Rap1 may also regulate proliferation, differentiation and survival through downstream effectors including B-Raf, PI3K, RalGEF and phospholipases (PLCs) (1-4). Rap1 and Rap2 are not fuctionally redundant as they perform overlapping but distinct functions (5). Recent research indicates that Rap2 regulates Dsh subcellular localization and is required for Wnt signaling in early development (6).
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Bos, J. et al. (2001) Nat. Rev. Mol. Cell Biol. 2, 369-377.
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Caron, E. (2003) J. Cell Sci. 116, 435-440.
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Song, C. et al. (2002) Oncogene 21, 8105-8113.
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Rong, R. et al. (2003) J. Biol. Chem. 278, 52497-52503.
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Taira, K. et al. (2004) J. Biol. Chem. 279, 49488-49496.
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Choi, S. and Han, J. (2005) EMBO J. 24, 985-996.
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