Apoptosis and Autophagy
Phospho-PAR-4 (Thr163) Antibody
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| CSTコード |
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| #2329S | 100 μL | 57,000 | |
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PAR-4抗体製品一覧
2329 の推奨プロトコール
最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。
注：各製品に最適化されたプロトコールをリンクしています。
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2329:
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Western Blotting
| 用途 (希釈倍率) | |
| ウェスタンブロッティング (1:1,000) |
| 特異性・感度 | |
| 内在性レベルのThr163 がリン酸化されたPAR-4 タンパク質を検出します。 (Thr163 はヒトの配列によるものでラットのThr155、マウスのThr156 周辺と同じ配列です) |
| 使用抗原 | |
| ヒトのPAR-4 タンパク質のThr163 周辺領域 (合成ペプチド) |
| ※括弧付きの動物種は、配列が100%相同であるため反応すると推定されます。 |
Western Blotting
Western blot analysis of extracts from HeLa cells, untreated or forskolin-treated (30 μM), using Phospho-PAR-4 (Thr163) Antibody (upper) or total PAR-4 Antibody #2328 (lower).
Western Blotting
Western blot analysis of extracts from HeLa cells, untreated or treated with Calyculin A (100 nM, 5 minutes), using Phospho-PAR-4 (Thr163) Antibody (upper) or total PAR-4 Antibody #2328 (lower).
PAR-4 (prostate apoptosis response-4) was identified as a protein that is upregulated in prostate tumor cells undergoing apoptosis (1). Additionally, in parallel studies PAR-4 was found in the yeast two-hybrid system to bind to the Wilms' tumor suppressor protein WT1 and may modulate WT1-medated transcriptional activation (2). PAR-4 contains a leucine zipper domain and a death domain and has been implicated as an effector of apoptosis during tumorigenesis as well as in neurodegenerative disorders (3,4). PAR-4 is widely expressed in normal tissues but can be downregulated in some tumor types. The mechanism of PAR-4 mediated apoptosis regulation appears to be complex and dependent on the cellular context. Studies have indicated roles for PAR-4 in activation of the Fas-FADD-caspase-8 pathway as well as inhibition of the NF-κB pro-survival pathway (5-7). Its activity is likely to depend on the cellular context and post-translational modifications. For instance, phosphorylation of PAR-4 by Akt prevents its nuclear translocation thereby promoting cell surivival (8). In contrast, phoshorylation of rat PAR-4 at T155 by PKA appears to positively regulate its apoptotic activity (9).
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Sells, S.F. et al. (1997) Mol. Cell Biol. 17, 3823-3832.
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Johnstone, R.W. et al. (1996) Mol. Cell Biol. 16, 6945-6956.
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Guo, Q. et al. (1998) Nat. Med. 4, 957-962.
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El-Guendy, N. and Rangnekar, V.M. (2003) Exp. Cell Res. 283, 51-66.
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Chakraborty, M. et al. (2001) Cancer Res. 61, 7255-7263.
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Díaz-Meco, M.T. et al. (1996) Cell 86, 777-786.
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Diaz-Meco, M.T. et al. (1999) J. Biol. Chem. 274, 19606-79612.
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Goswami, A. et al. (2005) Mol. Cell 20, 33-44.
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Gurumurthy, S. et al. (2005) Mol. Cell Biol. 25, 1146-1161.
PhosphoSitePlus®：
PAR-4