Antibody Sampler Kits
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| CSTコード | 包装 | 希望納入価格 (円) |
国内在庫  2012年2月3日18時20分 現在 |
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| #2330S | 1 Kit | 115,000 | ログインすると国内在庫状況がご確認いただけます。
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2330 の推奨プロトコール 最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。 注：各製品に最適化されたプロトコールをリンクしています。 |
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- 2696:
- Western Blotting
- 4431:
- Western Blotting
- 4432:
- Immunoprecipitation Western Blotting
- 9421:
- Immunoprecipitation Western Blotting
- 9422:
- Immunoprecipitation Western Blotting
- 9427:
- Western Blotting
- 9431:
- Western Blotting
- 9434:
- Flow Immunoprecipitation Western Blotting
| キット内容 | 容量 | 用途 | 種交差性 | 検出タンパク質の分子量 | Isotype |
|---|---|---|---|---|---|
| Phospho-A-Raf (Ser299) Antibody #4431 | 40 µl | W | H M R | 68 | Rabbit |
| A-Raf Antibody #4432 | 40 µl | W IP | H M R | 68 | Rabbit |
| Phospho-c-Raf (Ser338) (56A6) Rabbit mAb #9427 | 40 µl | W | H M R Mk | 74 | Rabbit IgG |
| Phospho-c-Raf (Ser289/296/301) Antibody #9431 | 40 µl | W | H M (R) | 74 | Rabbit |
| Phospho-c-Raf (Ser259) Antibody #9421 | 40 µl | W IP | H M R X (C) | 74 | Rabbit |
| c-Raf Antibody #9422 | 40 µl | W IP | H M R Mk | 65 to 75 | Rabbit |
| Phospho-B-Raf (Ser445) Antibody #2696 | 40 µl | W | H M R Mk (C) (Dg) | 86 | Rabbit |
| B-Raf (L12G7) Mouse mAb #9434 | 40 µl | W IP F | H M R Mk Dm | 86 | Mouse IgG1 |
| Anti-rabbit IgG, HRP-linked Antibody #7074 | 100 µl | Goat | |||
| Anti-mouse IgG, HRP-linked Antibody #7076 | 100 µl | Horse |
| 貯法 | |
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| -20℃ |
| ※括弧付きの動物種は配列が100%相同であるため反応すると推定されます。 |
| 社内データ |
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Western Blotting
Western blot analysis of extracts from Raji and HeLa cells, untreated or TPA-treated (200 nM, 30 minutes) using Phospho-B-Raf (Ser445) Antibody #2696 (top) and total B-Raf Antibody (bottom).
Western Blotting
Western blot analysis of extracts from Raji and HeLa cells, untreated or TPA-treated (30 minutes), using Phospho-A-Raf (Ser299) Antibody #4431 (upper) or A-Raf Antibody #4432 (lower).
Western Blotting
Western blot analysis of extracts from HT29, NIH/3T3 and C6 cell lysates using A-Raf Antibody #4432.
Western Blotting
Western blot analysis of recombinant Myc-tagged c-Raf protein, wildtype (lanes 1 and 3) and S259A mutant (lanes 2 and 4), using Phospho-Raf (Ser259) Antibody #9421 or a Myc antibody (provided by Dr. Guri Tzivion, Massachusetts General Hospital).
Western Blotting
Western blot analysis of extracts from HeLa cells, untreated or TPA-treated, using Phospho-c-Raf (Ser259) Antibody #9421 (upper), or a total c-Raf antibody (lower).
Western Blotting
Western blot analysis of extracts from 293 cells, untreated or TPA-treated (200 nM for 30 minutes), using c-Raf Antibody #9422 (lower) or Phospho-c-Raf (Ser338) Rabbit mAb #9427 (upper).
Western Blotting
Western blot analysis of extracts from NIH3T3, HeLa and COS cells, untreated or treated with TPA using Phospho-c-Raf (Ser338) (56A6) Rabbit mAb #9427.
Western Blotting
Western blot analysis of extracts from untreated or TPA-treated 293 and NIH/3T3 cells using Phospho-c-Raf (Ser289/296/301) Antibody #9431.
Western Blotting
Western blot analysis of extracts from K-562, HeLa, C1C12, NBT-II and COS cells using B-Raf (L12G7) Mouse mAb #9434.
| バックグラウンド |
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A-Raf, B-Raf and c-Raf (Raf-1) are the main effectors recruited by GTP-bound Ras to activate the MEK-MAP kinase pathway (1). Activation of c-Raf is the best understood and involves phosphorylation at multiple activating sites including Ser338, Tyr341, Thr491, Ser494, Ser497 and Ser499 (2). p21-activated protein kinase (PAK) has been shown to phosphorylate c-Raf at Ser338 and the Src family phosphorylates Tyr341 to induce c-Raf activity (3,4). Ser338 of c-Raf corresponds to similar sites in A-Raf (Ser299) and B-Raf (Ser445), although this site is constitutively phosphorylated in B-Raf (5). Inhibitory 14-3-3 binding sites on c-Raf (Ser259 and Ser621) can be phosphorylated by Akt and AMPK, respectively (6,7). While A-Raf, B-Raf and c-Raf are similar in sequence and function, differential regulation has been observed (8). Of particular interest, B-Raf contains three consensus Akt phosphorylation sites (Ser364, Ser428 and Thr439) and lacks a site equivalent to Tyr341 of c-Raf (8,9). The B-Raf mutation V600E results in elevated kinase activity and is commonly found in malignant melanoma (10). Six residues of c-Raf (Ser29, Ser43, Ser289, Ser296, Ser301 and Ser642) become hyperphosphorylated in a manner consistent with c-Raf inactivation. The hyperphosphorylation of these six sites is dependent on downstream MEK signaling and renders c-Raf unresponsive to subsequent activation events (11).
- Avruch, J. et al. (1994) Trends Biochem. Sci. 19, 279-283.
- Chong, H. et al. (2001) EMBO J. 20, 3716-3727.
- King, A.J. et al. (1998) Nature 396, 180-183.
- Fabian, J.R. et al. (1993) Mol. Cell Biol. 13, 7170-7179.
- Mason, C.S. et al. (1999) EMBO J. 18, 2137-2148.
- Zimmermann, S. and Moelling, K. (1999) Science 286, 1741-1744.
- Sprenkle, A.B. et al. (1997) FEBS Lett. 403, 254-258.
- Marais, R. et al. (1997) J. Biol. Chem. 272, 4378-4383.
- Guan, K.L. et al. (2000) J. Biol. Chem. 275, 27354-27359.
- Davies, H. et al. (2002) Nature 417, 949-954.
- Dougherty, M.K. et al. (2005) Mol. Cell 17, 215-224.
| 使用例 | |
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| 製品をご使用いただいて研究を発表されましたら、ぜひお知らせください。 |
本製品は試験研究用です。