PI3K / Akt Signaling
- PI3K / Akt Signaling
PhosphoSitePlus®：
Akt1
- 推奨プロトコール
推奨アプリケーションで優れたパフォーマンスを発揮するXP®モノクローナル抗体をお試しください : 4075
Phospho-Akt (Ser473) (193H12) Rabbit mAb (Alexa Fluor® 647 Conjugate) |
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| CSTコード | 包装 | 希望納入価格 (円) |
国内在庫  2012年2月9日15時20分 現在 |
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| #2337S | 100 μL (50 tests) | 61,000 | ログインすると国内在庫状況がご確認いただけます。
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2337 の推奨プロトコール 最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。 注：各製品に最適化されたプロトコールをリンクしています。 |
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- 2337:
- Flow
| 用途 (希釈倍率) | |
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| フローサイトメトリー (1:50) |
| 種交差性 | |
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| ヒト、マウス、サル |
| 特異性・感度 | |
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| 内在性レベルのSer473 がリン酸化されたAkt タンパク質を検出します。 |
| 使用抗原 | |
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| マウスのAkt タンパク質のSer473 周辺領域 (合成リン酸化ペプチド) |
| 抗体の由来 | |
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| ウサギ |
| 貯法 | |
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| 4℃ |
| Alexa Fluor®はMolecular Probes, Inc. の登録商標です。 |
| 社内データ |
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推奨アプリケーションで優れたパフォーマンスを発揮するXP®モノクローナル抗体をお試しください : 4075
Flow Cytometry
Flow cytometric analysis of Jurkat cells, untreated (green) or LY294002 and wortmannin treated (blue), using Phospho-Akt (Ser473) (193H12) Rabbit mAb (Alexa Fluor® 647 Conjugate).
| バックグラウンド |
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Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis (1-3). This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase (2,3). Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 (4) and by phosphorylation within the carboxy terminus at Ser473. The previously elusive PDK2 responsible for phosphorylation of Akt at Ser473 has been identified as mammalian target of rapamycin (mTOR) in a rapamycin-insensitive complex with rictor and Sin1 (5,6). Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including Bad (7), forkhead transcription factors (8), c-Raf (9), and caspase-9. PTEN phosphatase is a major negative regulator of the PI3 kinase/Akt signaling pathway (10). LY294002 is a specific PI3 kinase inhibitor (11). Another essential Akt function is the regulation of glycogen synthesis through phosphorylation and inactivation of GSK-3α and β (12,13). Akt may also play a role in insulin stimulation of glucose transport (12). In addition to its role in survival and glycogen synthesis, Akt is involved in cell cycle regulation by preventing GSK-3β-mediated phosphorylation and degradation of cyclin D1 (14) and by negatively regulating the cyclin dependent kinase inhibitors p27 Kip (15) and p21 Waf1/CIP1 (16). Akt also plays a critical role in cell growth by directly phosphorylating mTOR in a rapamycin-sensitive complex containing raptor (17). More importantly, Akt phosphorylates and inactivates tuberin (TSC2), an inhibitor of mTOR within the mTOR-raptor complex (18,19).
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- Burgering, B.M. and Coffer, P.J. (1995) Nature 376, 599-602.
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- Zimmermann, S. and Moelling, K. (1999) Science 286, 1741-4.
- Cantley, L.C. and Neel, B.G. (1999) Proc Natl Acad Sci USA 96, 4240-5.
- Vlahos, C.J. et al. (1994) J Biol Chem 269, 5241-8.
- Hajduch, E. et al. (2001) FEBS Lett 492, 199-203.
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- Diehl, J.A. et al. (1998) Genes Dev 12, 3499-511.
- Gesbert, F. et al. (2000) J Biol Chem 275, 39223-30.
- Zhou, B.P. et al. (2001) Nat Cell Biol 3, 245-52.
- Navé, B.T. et al. (1999) Biochem J 344 Pt 2, 427-31.
- Inoki, K. et al. (2002) Nat Cell Biol 4, 648-57.
- Manning, B.D. et al. (2002) Mol Cell 10, 151-62.
| 使用文献 |
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- Mahajan, K. et al. (2010) PLoS One 5, e9646. Applications: Flow Cytometry
本製品は試験研究用です。