Cell Cycle / Checkpoint Control
| CSTコード |
包装 |
希望納入価格（円） |
国内在庫  |
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| #2341S | 100 μL | 57,000 | |
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| #2341L | 300 μL | 137,000 | |
Chk1抗体製品一覧
推奨プロトコール
最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。
注：各製品に最適化されたプロトコールをリンクしています。
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2341:
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Western Blotting
| 用途（希釈倍率） | |
| ウェスタンブロッティング（1:1,000） |
| 特異性・感度 | |
| 内在性レベルのSer345 がリン酸化されたChk1 タンパク質を検出します。他の部位がリン酸化されたChk1 タンパク質とは交差しません。 |
| 使用抗原 | |
| ヒトのChk1 タンパク質のSer345 周辺領域（合成リン酸化ペプチド） |
Western Blotting
Western blot analysis of extracts from COS cells treated with UV or MMS for the indicated times using Phospho-Chk1 (Ser345) Antibody.
Western Blotting
Western blot analysis of extracts from C6 and HeLa cells treated with UV, MMS or HU as indicated using Phospho-Chk1 (Ser345) Antibody.
Chk1 kinase acts downstream of ATM/ATR kinase to play an important role in DNA damage checkpoint control, embryonic development and tumor suppression (1). Activation of Chk1 involves phosphorylation of Ser317 and Ser345 and occurs in response to blocked DNA replication and certain forms of genotoxic stress (2). Phosphorylation at Ser 345 serves to localize Chk1 to the nucleus (3) following checkpoint acitvation while recently phosphorylation at Ser 317 along with site-specific phosphorylation of PTEN was shown to allow for reentry into the cell cycle following stalled DNA replication. (4). Chk1 exerts it checkpoint mechanism on the cell cycle in part by regulating the cdc25 family of phosphatases. Chk1 phosphorylation of cdc25A targets it for proteolysis and inhibits it's activity though 14-3-3 binding. (5). Activated Chk1 can inactivate cdc25C via phosphorylation at Ser216, blocking the activation of cdc2 and transition into mitosis (6). Also, centrosomal Chk1 has been shown to phosphorylate cdc25B inhibiting its activation of CDK1-cyclin B1 and thus mitotic spindle formation and chromatin condensation (7). Furthermore, Chk1 plays a role in spindle checkpoint function through regulation of Aurora B and BubR1 (8). Chk1 has emerged as a drug target for cancer therapy as its inhibition leads to cell death in many cancer cell lines (9).
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Liu, Q. et al. (2000)
Genes Dev
14, 1448-59.
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Zhao, H. and Piwnica-Worms, H. (2001)
Mol Cell Biol
21, 4129-39.
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Jiang, K. et al. (2003)
J Biol Chem
278, 25207-17.
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Martin, S.A. and Ouchi, T. (2008)
Mol Cancer Ther
7, 2509-16.
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Chen, M.S. et al. (2003)
Mol Cell Biol
23, 7488-97.
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Zeng, Y. et al. (1998)
Nature
395, 507-10.
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Löffler, H. et al. (2006)
Cell Cycle
5, 2543-7.
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Zachos, G. et al. (2007)
Dev Cell
12, 247-60.
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Garber, K. (2005)
J Natl Cancer Inst
97, 1026-8.
- Cliby, W. A. et al. (2002) S phase and G2 arrests induced by topoisomerase I poisons are dependent on ATR kinase function. J. Biol. Chem. 277 (2), 1599-1606. Applications: Western Blotting
- Castedo, M. et al. (2004) The cell cycle checkpoint kinase Chk2 is a negative regulator of mitotic catastrophe. Oncogene 23, 4353-4361. Applications: Western Blotting
- d'Adda di Fagagna, F. et al. (2003) A DNA damage checkpoint response in telomere-initiated senescence. Nature 426, 194-198. Applications: Western Blotting
- Fang, Y. et al. (2004) ATR functions as a gene dosage-dependent tumor suppressor on a mismatch repair-deficient background. EMBO J. 23, 3164-3174. Applications: Western Blotting
- Shibata, Y. and Nakamura, T. (2002) Defective flap endonuclease 1 activity in mammalian cells is associated with impaired DNA repair and prolonged S phase delay. J. Biol. Chem. 277, 746-754. Applications: Western Blot
- Deming , P. B. et al. (2001) The human decatenation checkpoint. Proc. Natl. Acad. Sci. USA 98, 12044-12049. Applications: Western Blot
