Vesicle Trafficking
Clathrin Heavy Chain (P1663) Antibody
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イイネ!(2)
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| CSTコード |
包装 |
希望納入価格 (円) |
国内在庫  |
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| #2410S | 100 μL | 46,000 | |
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Clathrin Heavy Chain XP®モノクローナル抗体 | Clathrin Heavy Chain抗体製品一覧
2410 の推奨プロトコール
最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。
注:各製品に最適化されたプロトコールをリンクしています。
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2410:
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Immunofluorescence
Western Blotting
| 用途(希釈倍率) | |
| ウェスタンブロッティング(1:1,000)、免疫蛍光細胞染色(IF-IC)(1:400) |
| 特異性・感度 | |
| 内在性レベルのClathrin タンパク質を検出します。 |
| 使用抗原 | |
| ヒトのClathrin タンパク質のPro1663 周辺領域(合成ペプチド) |
Western Blotting

Western blot analysis of extracts from SHSY5Y, Neuro2A, PC12 and COS cells, using Clathrin Heavy Chain (P1663) Antibody.
IF-IC

Confocal immunofluorescent analysis of HeLa cells labeled with Clathrin Heavy Chain (P1663) Antibody (green). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).
Clathrin-coated vesicles provide for the intracellular transport of cargo proteins following endocytosis and during multiple vesicle trafficking pathways. Vesicles form at specialized areas of the cell membrane where clathrin and associated proteins form clathrin-coated pits. Invagination of these cell membrane-associated pits internalizes proteins and forms an intracellular clathrin-coated vesicle (1,2). Clathrin is the most abundant protein in these vesicles and is present as a basic assembly unit called a triskelion. Each clathrin triskelion is composed of three clathrin heavy chains and three clathrin light chains. Clathrin heavy chain proteins are composed of several functional domains, including a carboxy-terminal region that permits interaction with other heavy chain proteins within a triskelion, and a globular amino-terminal region that associates with other vesicle proteins (2). Adaptor proteins, such as AP2, epsin and EPS15, are responsible for the recruitment of vesicle proteins to sites of pit formation and the assembly of the clathrin-coated vesicle. Following vesicle invagination, the GTPase dynamin constricts the neck of the nascent vesicle to complete formation of the free, cytosolic vesicle (3,4).
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Rodriguez-Boulan, E. et al. (2005) Nat. Rev. Mol. Cell Biol. 6, 233-247.
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Mousavi, S.A. et al. (2004) Biochem. J. 377, 1-16.
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Rappoport, J.Z. et al. (2004) Traffic 5, 327-237.
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Brett, T.J. and Traub, L.M. (2006) Curr. Opin. Cell Biol. 18, 395-406.