Angiogenesis
Phospho-VEGF Receptor 2 (Tyr1212) (11A3) Rabbit mAb
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| #2477S | 100 μL | 57,000 | |
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VEGFR2抗体製品一覧
2477 の推奨プロトコール
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2477:
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Western Blotting
| 用途 (希釈倍率) | |
| ウェスタンブロッティング (1:1,000) |
| 特異性・感度 | |
| トランスフェクトレベルのTyr1212 がリン酸化されたVEGFR-2 タンパク質を検出します。他のリン酸化受容体型チロシンキナーゼとは交差しません。 |
| 使用抗原 | |
| ヒトのVEGFR-2 タンパク質のTy1214 周辺領域 (合成リン酸化ペプチド) |
Western Blotting
Western blot analysis of recombinant human GST-VEGF Receptor 2 (Val789-Val1356), untreated or λ phosphatase-treated, using Phospho-VEGF Receptor 2 (Tyr1212) (11A3) Rabbit mAb (upper) and VEGF Receptor 2 Antibody #2472 (lower).
IP
Phospho-VEGF Receptor 2 (Tyr1212) (11A3) Rabbit mAb specifically binds to phosphorylated VEGFR2, but not other phosphorylated tyrosine kinases. Western blot analysis of extracts from cells expressing different activated tyrosine kinase proteins, using Phospho-VEGF Receptor-2 (Tyr1212) (11A3) Rabbit mAb (upper) and Phospho-Tyrosine mAb (P-Tyr-100) #9411 (lower). CKR/PAE cells (lanes 12 and 13) express chimeric receptors containing human CSF-1 extracellular binding domain/mouse VEGF receptor-2 intracellular domain (5). CSF-1 stimulates phosphorylation of Tyr1212 of intracellular VEGF receptor-2 domain (lane 12) , which was specifically detected by Phospho-VEGF Receptor-2 (Tyr1212) (11A3) Rabbit mAb.
Vascular endothelial growth factor receptor 2 (VEGFR2, KDR, Flk-1) is a major receptor for VEGF-induced signaling in endothelial cells. Upon ligand binding, VEGFR2 undergoes autophosphorylation and becomes activated (1). Major autophosphorylation sites of VEGFR2 are located in the kinase insert domain (Tyr951/996) and in the tyrosine kinase catalytic domain (Tyr1054/1059) (2). Activation of the receptor leads to rapid recruitment of adaptor proteins, including Shc, Grb2, PI-3 kinase, Nck and the protein tyrosine phosphatases SHP-1 and SHP-2 (3). The phosphorylation of Tyr1212 provides a docking site for Grb2 binding and phospho-Tyr1175 binds with the p85 subunit of PI-3 kinase and PLCγ, as well as Shb (1,4,5). Signaling from VEGFR2 is necessary for the execution of VEGF-stimulated proliferation, chemotaxis and sprouting, as well as survival of cultured endothelial cells in vitro and angiogenesis in vivo (6-8).
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Meyer, M. et al. (1999) EMBO J 18, 363-74.
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Dougher-Vermazen, M. et al. (1994) Biochem Biophys Res Commun 205, 728-38.
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Kroll, J. and Waltenberger, J. (1997) J Biol Chem 272, 32521-7.
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Takahashi, T. et al. (2001) EMBO J 20, 2768-78.
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Holmqvist, K. et al. (2004) J Biol Chem 279, 22267-75.
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Karkkainen, M.J. and Petrova, T.V. (2000) Oncogene 19, 5598-605.
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Rahimi, N. et al. (2000) J Biol Chem 275, 16986-92.
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Claesson-Welsh, L. (2003) Biochem Soc Trans 31, 20-4.
PhosphoSitePlus®：
VEGFR-2