Tyrosine Kinases / Adaptors
- Tyrosine Kinases / Adaptors
PhosphoSitePlus®：
VEGFR-2
- 推奨プロトコール
VEGF Receptor 2 (55B11) Rabbit mAb |
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| CSTコード | 包装 | 希望納入価格 (円) |
国内在庫  2012年2月9日11時30分 現在 |
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| #2479S | 100 μL | 46,000 | ログインすると国内在庫状況がご確認いただけます。
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| #2479L | 300 μL | 111,000 |
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2479 の推奨プロトコール 最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。 注：各製品に最適化されたプロトコールをリンクしています。 |
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下記ステップについては、データシートの右側もあわせてご参照ください。
IHC-P: 抗体希釈液 / 抗原賦活化
| 用途 (希釈倍率) | |
|---|---|
| ウェスタンブロッティング (1:1,000)、免疫沈降 (1:100)、免疫組織染色 (パラフィン) (1:600)、免疫蛍光細胞染色 (IF-IC) (1:200)、免疫蛍光染色 (IF-F) (1:200) |
| 種交差性 | |
|---|---|
| ヒト、マウス |
| 特異性・感度 | |
|---|---|
| 内在性レベルのVEGF Receptor 2 タンパク質を検出します。他のファミリータンパク質とは交差しません。 |
| 検出タンパク質の分子量 | |
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| 210 kDa、230 kDa |
| 使用抗原 | |
|---|---|
| ヒトのVEGF Receptor 2 のC末端側の150アミノ酸残基を含む組み換えタンパク質 |
| 抗体の由来 | |
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| ウサギ |
| 貯法 | |
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| -20℃ |
| 社内データ |
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Western Blotting
Western blot analysis of extracts from various cell lines and primary cell cultures, using VEGF Receptor 2 (55B11) Rabbit mAb. PAE/CKR cells overexpress chimeric receptors containing human CSF-1 extracellular binding domain/mouse VEGF receptor 2 intracellular domains (Rahimi, N. et al. [2000] J. Biol. Chem. 275, 16986-16992). PAE/VEGFR1 cells overexpress human VEGF receptor 1.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human astrocytoma, using VEGF Receptor 2 (55B11) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded breast angiosarcoma, using VEGF Receptor 2 (55B11) Rabbit mAb (left). A serial section is stained for CD31 (PECAM-1), an endothelial cell marker (right).
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded HUVEC cells, using VEGF Receptor 2 (55B11) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human renal adenocarcinoma, using VEGF Receptor 2 (55B11) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded HT-29 xenograft, using VEGF Receptor 2 (55B11) Rabbit mAb. Note staining of mouse blood vessels.
IF-IC
Confocal immunofluorescent images of HUVEC cells untreated (left) or stimulated with Vascular Endothelial Growth Factor (VEGF) #9943 (right) and labeled with Phospho-VEGF Receptor 2 (Tyr1175) (19A10) Rabbit mAb #2478 (top, green) and VEGF Receptor 2 (55B11) Rabbit mAb (bottom, green). Blue pseudocolor = DRAQ5® (fluorescent DNA dye).
IF-F
Confocal immunofluorescent analysis of mouse pancreas using VEGF Receptor 2 (55B11) Rabbit mAb (green) and S6 Ribosomal Protein (54D2) Mouse mAb #2317 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
| バックグラウンド |
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Vascular endothelial growth factor receptor 2 (VEGFR2, KDR, Flk-1) is a major receptor for VEGF-induced signaling in endothelial cells. Upon ligand binding, VEGFR2 undergoes autophosphorylation and becomes activated (1). Major autophosphorylation sites of VEGFR2 are located in the kinase insert domain (Tyr951/996) and in the tyrosine kinase catalytic domain (Tyr1054/1059) (2). Activation of the receptor leads to rapid recruitment of adaptor proteins, including Shc, Grb2, PI-3 kinase, Nck and the protein tyrosine phosphatases SHP-1 and SHP-2 (3). The phosphorylation of Tyr1212 provides a docking site for Grb2 binding and phospho-Tyr1175 binds with the p85 subunit of PI-3 kinase and PLCγ, as well as Shb (1,4,5). Signaling from VEGFR2 is necessary for the execution of VEGF-stimulated proliferation, chemotaxis and sprouting, as well as survival of cultured endothelial cells in vitro and angiogenesis in vivo (6-8).
- Meyer, M. et al. (1999) EMBO J 18, 363-74.
- Dougher-Vermazen, M. et al. (1994) Biochem Biophys Res Commun 205, 728-38.
- Kroll, J. and Waltenberger, J. (1997) J Biol Chem 272, 32521-7.
- Takahashi, T. et al. (2001) EMBO J 20, 2768-78.
- Holmqvist, K. et al. (2004) J Biol Chem 279, 22267-75.
- Karkkainen, M.J. and Petrova, T.V. (2000) Oncogene 19, 5598-605.
- Rahimi, N. et al. (2000) J Biol Chem 275, 16986-92.
- Claesson-Welsh, L. (2003) Biochem Soc Trans 31, 20-4.
| 使用文献 |
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- Antonescu, C.R. et al. (2009) Cancer Res Applications: IF-IC (In Cells) IHC-P (paraffin) Western Blotting
- Kitamura, T. et al. (2008) Nat Cell Biol 10, 329-37. Applications: Western Blotting
本製品は試験研究用です。