Glucose / Energy Metabolism
SirT1 (C14H4) Rabbit mAb
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| CSTコード |
包装 |
希望納入価格 (円) |
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| #2496S | 100 μL | 46,000 | |
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SirT1抗体製品一覧
2496 の推奨プロトコール
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推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。
注：各製品に最適化されたプロトコールをリンクしています。
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2496:
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Immunoprecipitation
Western Blotting
| 用途（希釈倍率） | |
| ウエスタンブロッティング（1:1,000）、免疫沈降（1:25） |
| 特異性・感度 | |
| 内在性レベルのSirT1 タンパク質を検出します。他のSirtuin タンパク質とは交差しません。 |
| 使用抗原 | |
| ヒトのSirT1 タンパク質のC 末端由来の配列（合成ペプチド） |
Western Blotting
Western blot analysis of extracts from various cell types using SirT1 (C14H4) Rabbit mAb.
The Silent Information Regulator (SIR2) family of genes is a highly conserved group of genes that encode nicotinamide adenine dinucleotide (NAD)-dependent protein deacetylases, also known as class III histone deacetylases. The first discovered and best characterized of these genes is Saccharomyces cerevisiae SIR2, which is involved in silencing of mating type loci, telomere maintenance, DNA damage response, and cell aging (1). SirT1, the mammalian ortholog of Sir2, is a nuclear protein implicated in the regulation of many cellular processes, including apoptosis, cellular senescence, endocrine signaling, glucose homeostasis, aging, and longevity. Targets of SirT1 include acetylated p53 (2,3), p300 (4), Ku70 (5), forkhead (FoxO) transcription factors (5,6), PPARγ (7), and the PPARγ coactivator-1α (PGC-1α) protein (8). Deacetylation of p53 and FoxO transcription factors represses apoptosis and increases cell survival (2,3,5,6). Deacetylation of PPARγ and PGC-1α regulates the gluconeogenic/glycolytic pathways in the liver and fat mobilization in white adipocytes in response to fasting (7,8). SirT1 deacetylase activity is inhibited by nicotinamide and activated by resveratrol. In addition, SirT1 activity may be regulated by phosphorylation, since it is phosphorylated on Ser27 and Ser47 in vivo, however, the function of these phosphorylation sites has not yet been determined (9).
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Guarente, L. (1999) Nat. Genet. 23, 281-285.
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Vaziri, H. et al. (2001) Cell 107, 149-159.
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Luo, J. et al. (2001) Cell 107, 137-148.
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Bouras, T. et al. (2005) J. Biol. Chem. 280, 10264-10276.
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Brunet, A. et al. (2004) Science 303, 2011-2015.
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Motta, M.C. et al. (2004) Cell 116, 551-563.
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Picard, F. et al. (2004) Nature 429, 771-776.
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Rodgers, J.T. et al. (2005) Nature 434, 113-118.
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Beausoleil, S.A. et al. (2004) Proc. Natl. Acad. Sci. USA 101, 12130-12135.
PhosphoSitePlus®：
SIRT1