Wnt / Hedgehog / Notch
TCF4 (C9B9) Rabbit mAb
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| CSTコード |
包装 |
希望納入価格 (円) |
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| #2565S | 100 μL | 46,000 | |
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TCF4抗体製品一覧
2565 の推奨プロトコール
最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。
注：各製品に最適化されたプロトコールをリンクしています。
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2565:
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ChIP Agarose
ChIP Magnetic
Immunoprecipitation
Western Blotting
| 用途（希釈倍率） | |
| ウェスタンブロッティング（1:1,000）、免疫沈降（1:50）、ChIP(1:50) |
| 特異性・感度 | |
| 内在性レベルのTCF4 タンパク質を検出します。 |
| 検出タンパク質の分子量 | |
| 58 kDa、79 kDa |
| 使用抗原 | |
| ヒトのTCF4 タンパク質のGlu81 周辺領域（合成ペプチド） |
| ※括弧付きの動物種は配列が100%相同であるため反応すると推定されます。 |
Western Blotting
Western blot analysis of total cell lysates from HCT116, DLD1 and HepG2 cells using TCF4 (C9B9) Rabbit mAb.
Chromatin IP
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 HCT 116 cells and either 10 μl of TCF4 (C9B9) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human CaMK2D Intron 3 Primers #5111, human c-Myc promoter primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
LEF1 and TCF are members of the high mobility group (HMG) DNA binding protein family of transcription factors that consists of the following: Lymphoid Enhancer Factor 1 (LEF1), T Cell Factor 1 (TCF1), TCF3, and TCF4 (1). LEF1 and TCF1 were originally identified as important factors regulating early lymphoid development (2) and act downstream in Wnt signaling. LEF1 and TCF bind to Wnt response elements to provide docking sites for β-catenin, which translocates to the nucleus to promote the transcription of target genes upon activation of Wnt signaling (3). LEF1 and TCF are dynamically expressed during development and aberrant activation of the Wnt signaling pathway is involved in many types of cancers including colon cancer (4,5).
TCF4, also known as TCF7L2, is expressed widely during development. Gene targeting studies indicate that TCF4 is required to maintain the crypt stem cells of the small intestine (6,7). TCF4 has several slicing isoforms which are expressed differentially in tissues and during cancer progression (8,9). Studies also indicate that a variant of the TCF4 gene confers an increased risk of type 2 diabetes (10).
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Waterman, M.L. (2004) Cancer Metastasis Rev. 23, 41-52.
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Schilham, M.W. and Clevers, H. (1998) Semin. Immunol. 10, 127-132.
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Brantjes, H. et al. (2002) Biol. Chem. 383, 255-261.
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Reya, T. and Clevers, H. (2005) Nature 434, 843-850.
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Logan, C.Y. and Nusse, R. (2004) Annu. Rev. Cell Dev. Biol. 20, 781-810.
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Cho, E.A. and Dressler, G.R. (1998) Mech. Dev. 77, 9-18.
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Korinek, V. et al. (1998) Nat. Genet. 19, 379-383.
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Howng, S.L. et al. (2004) Int. J. Oncol. 25, 1685-1692.
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Shiina, H. et al. (2003) Clin. Cancer Res. 9, 2121-2132.
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Grant, S.F. et al. (2006) Nat. Genet. 38, 320-323.
PhosphoSitePlus®：
TCF7L2