Cytoskeletal Signaling
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PhosphoSitePlus®：
| CSTコード | 包装 | 希望納入価格 (円) |
国内在庫  2012年2月7日15時25分 現在 |
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| #2602S | 100 μL | 46,000 | ログインすると国内在庫状況がご確認いただけます。
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| #2602P | 40 μL for Custom Sampler Kit |  Custom Antibody Sampler Kitの構成品を選択できます。 5本以上を選択し、ページ右上のCartから製品確定書を発行してください。 尚、構成品の単品販売は致しておりません。 |
シグナル伝達研究応援キャンペーン プレゼント *Pサイズのみ
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2602 の推奨プロトコール 最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。 注：各製品に最適化されたプロトコールをリンクしています。 |
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下記ステップについては、データシートの右側もあわせてご参照ください。
IHC-P: 抗体希釈液 / 抗原賦活化
| 用途 (希釈倍率) | |
|---|---|
| ウェスタンブロッティング (1:1,000)、免疫沈降 (1:50)、免疫組織染色 (パラフィン) (1:50) |
| 種交差性 | |
|---|---|
| ヒト、マウス、ラット、サル、モルモット |
| 特異性・感度 | |
|---|---|
| 内在性レベルのPAK1 タンパク質を検出します。PAK2、PAK3 タンパク質や他のPAK ファミリータンパク質とは交差しません。 |
| 検出タンパク質の分子量 | |
|---|---|
| 68 kDa |
| 使用抗原 | |
|---|---|
| ヒトのPAK1 タンパク質のN末端領域 (合成ペプチド) |
| 抗体の由来 | |
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| ウサギ |
| 貯法 | |
|---|---|
| -20℃ |
| 社内データ |
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Western Blotting
Western blot analysis of extracts from SH-SY5Y, C6 and NIH/3T3 cells, using PAK1 Antibody.
Western Blotting
Western blot analysis of extracts from HeLa cells transfected with 100 nM control siRNA #6201 (-) or PAK1 siRNA, using PAK1 Antibody #2602 and p42 MAP Kinase (Erk2) Antibody #9108. The PAK1 Antibody confirms silencing of PAK1 expression, and p42 MAP Kinase (Erk2) Antibody is used to control for loading and specificity of PAK1 siRNA.
IP
Immunoprecipitation of PAK1 from C6 and SH-SY5Y cells followed by Western blot analysis, using PAK1 Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing cytoplasmic localization, using PAK1 Antibody.
| バックグラウンド |
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The p21-activated kinase (PAK) family of serine/threonine kinases is engaged in multiple cellular processes, including cytoskeletal reorganization, MAPK signaling, apoptotic signaling, control of phagocyte NADPH oxidase, and growth factor-induced neurite outgrowth (1,2). Several mechanisms that induce PAK activity have been reported. Binding of Rac/Cdc42 to the CRIB (or PBD) domain near the amino terminus of PAK causes autophosphorylation and conformational changes in PAK (1). Phosphorylation of PAK1 at Thr423 by PDK induces activation of PAK1 (3). Several autophosphorylation sites have been identified, including Ser199 and Ser204 of PAK1 and Ser192 and Ser197 of PAK2 (4,5). Because the autophosphorylation sites are located in the amino-terminal inhibitory domain, it has been hypothesized that modification in this region prevents the kinase from reverting to an inactive conformation (6). Research indicates that phosphorylation at Ser144 of PAK1 or Ser139 of PAK3 (located in the kinase inhibitory domain) affects kinase activity (7). Phosphorylation at Ser21 of PAK1 or Ser20 of PAK2 regulates binding with the adaptor protein Nck (8). PAK4, PAK5, and PAK6 have lower sequence similarity with PAK1-3 in the amino-terminal regulatory region (9). Phosphorylation at Ser474 of PAK4, a site analogous to Thr423 of PAK1, may play a pivotal role in regulating the activity and function of PAK4 (10).
- Knaus, U.G. and Bokoch, G.M. (1998) Int. J. Biochem. Cell Biol. 30, 857-862.
- Daniels, R.H. et al. (1998) EMBO J. 17, 754-764.
- King, C.C. et al. (2000) J. Biol. Chem. 275, 41201-41209.
- Manser, E. et al. (1997) Mol. Cell. Biol. 17, 1129-1143.
- Gatti, A. et al. (1999) J. Biol. Chem. 274, 8022-8028.
- Lei, M. et al. (2000) Cell 102, 387-397.
- Chong, C. et al. (2001) J. Biol. Chem. 276, 17347-17353.
- Zhao, Z. et al. (2000) Mol. Cell. Biol. 20, 3906-3917.
- Abo, A. et al. (1998) EMBO J. 17, 6527-6540.
- Qu, J. et al. (2001) Mol. Cell. Biol. 21, 3523-3533.
| 使用文献 |
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- Rudrabhatla, R. S. et al. (2003) Modulation of Myosin Light-Chain Phosphorylation by p21-Activated Kinase 1 in Escherichia coli Invasion of Human Brain Microvascular Endothelial Cells. Infection and Immunity 71 (5), 2787-2797. Applications: Western Blotting
- Chu, P. C. et al. (2004) A Novel Role for p21-Activated Protein Kinase 2 in T Cell Activation. The Journal of Immunology 172, 7324-7334. Applications: Western Blotting
- Weber, D. S. et al. (2004) Phosphoinositide-Dependent Kinase 1 and p21-Activated Protein Kinase Mediate Reactive Oxygen Species-Dependent Regulation of Platelet-Derived Growth Factor-Induced Smooth Muscle Cell Migration. Circulation Research 94, 1219-1226. Applications: Western Blotting
- Harfouche, R. et al. (2005) FASEB J 19, 1728-30. Applications: Western Blotting
- Misra, U.K. et al. (2005) J Immunol 175, 2525-33. Applications: Western Blotting
- Nikolic, D.S. et al. (2011) Blood 118, 4841-52. Applications: Western Blotting
- Jung, J. et al. (2011) Oncogene 30, 2264-74. Applications: Western Blotting
本製品は試験研究用です。