Protein Folding and Stability
VCP (7F3) Rabbit mAb
|
イイネ！(0)
| 
|
| CSTコード |
包装 |
希望納入価格 (円) |
国内在庫  |
ご登録代理店情報 カスタマー情報にご登録いただいた代理店を表示しています。
ご登録代理店の変更は こちら。 |
| #2649S | 100 μL | 46,000 | |
|
VCP抗体製品一覧
2649 の推奨プロトコール
最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。
注：各製品に最適化されたプロトコールをリンクしています。
| | |
-
2649:
-
Western Blotting
| 用途 (希釈倍率) | |
| ウェスタンブロッティング (1:1,000) |
| 種交差性 | |
| ヒト、マウス、ラット、サル、(アフリカツメガエル、ゼブラフィッシュ、ウシ、ブタ、出芽酵母) |
| 特異性・感度 | |
| 内在性レベルのVCP タンパク質を検出します。 |
| 使用抗原 | |
| ヒトのVCP タンパク質の配列 (合成ペプチド) |
| ※括弧付きの動物種は、配列が100%相同であるため反応すると推定されます。 |
Western Blotting
Western blot analysis of extracts from HeLa, NIH/3T3, C6 and COS cells, using VCP (7F3) Rabbit mAb.
Valosin-containing protein (VCP) is a highly conserved and abundant 97 kDa protein that belongs to the AAA (ATPase associated with a variety of cellular activities) family of proteins. VCP assembles as a homo-hexamer, forming a ring with a channel at its center (1,2,3). VCP homo-hexamers associate with a variety of protein cofactors to form many distinct protein complexes, which act as chaperones to unfold proteins and transport them to specific cellular compartments or to the proteosome (4). These protein complexes participate in many cellular functions, including vesicle transport and fusion, fragmentation and reassembly of the golgi stacks during mitosis, nuclear envelope formation and spindle disassembly following mitosis, cell cycle regulation, DNA damage repair, apoptosis, B- and T-cell activation, NF-κB-mediated transcriptional regulation, endoplasmic reticulum (ER)-associated degradation and protein degradation (4). VCP appears to localize mainly to the endoplasmic reticulum; however, tyrosine phosphorylation is associated with relocalization to the centrosome during mitosis (5). In addition, following cellular exposure to ionizing radition, VCP is phosphorylated at Ser784 in an ATM-dependent manner and accumulates in the nucleus at sites of double-stranded DNA breaks (DSBs) (6). Exposure to other types of DNA damaging agents such as UV light, bleomycin or doxorubicin results in phosphorylation of VCP by ATR and DNA-PK in an ATM-independent manner (6).
-
DeLaBarre, B. and Brunger, A.T. (2003) Nat. Struct. Biol. 10, 856-863.
-
Huyton, T. et al. (2003) J. Struct. Biol. 144, 337-348.
-
Dreveny, I. et al. (2004) EMBO J. 23, 1030-1039.
-
Wang, Q. et al. (2004) J. Struct. Biol. 146, 44-57.
-
Madeo, F. et al. (1998) Mol. Biol. Cell 9, 131-141.
-
Livingstone, M. et al. (2005) Cancer Res. 65, 7533-7540.
PhosphoSitePlus®：
VCP