Jak/Stat Pathway
| CSTコード |
包装 |
希望納入価格（円） |
国内在庫  |
ご登録代理店情報 カスタマー情報にご登録いただいた代理店を表示しています。
ご登録代理店の変更は こちら。 |
| #2653S | 100 μL | 46,000 | |
|
Stat4抗体製品一覧
推奨プロトコール
最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。
注：各製品に最適化されたプロトコールをリンクしています。
| | |
-
2653:
-
ChIP Agarose
ChIP Magnetic
Western Blotting
| 用途（希釈倍率） | |
| ウェスタンブロッティング（1:1,000）、ChIP（1:25） |
| 特異性・感度 | |
| 内在性レベルのStat4 タンパク質を検出します。 |
| 使用抗原 | |
| Stat4 タンパク質のLys151 周辺領域（合成ペプチド） |
Western Blotting
Western blot analysis of extracts from ACHN, SR and Caki cell lines and from rat thymus using Stat4 (C46B10) Rabbit mAb.
Chromatin IP
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10
6
NK-92 cells starved of IL-2 overnight then treated with IL-12 (10ng/ml) for 4h and either 20 μl of Stat4 (C46B10) Rabbit mAb #2653 or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP™ Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human IRF-1 promoter primers, human PRF1 promoter primers, and SimpleChIP™ Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
The Jak-Stat signaling pathway is utilized by a large number of cytokines, growth factors and hormones (1). Receptor-mediated tyrosine phosphorylation of Jak family members triggers phosphorylation of Stat proteins resulting in their nuclear translocation, binding to specific DNA elements and subsequent activation of transcription. The remarkable range and specificity of responses regulated by the Stats is determined in part by the tissue-specific expression of different cytokine receptors, Jaks and Stats as well as by the combinatorial coupling of various Stat members to different receptors (2). Stat4 is predominantly expressed in the spleen, thymus and testis and has been most extensively investigated as the mediator of IL-12 responses (3-8).
-
Darnell, J.E. et al. (1994)
Science
264, 1415-1421.
-
Leonard, W.J. and O'Shea, J.J. (1998)
Annu. Rev. Immunol.
16, 293-322.
-
Zhong, Z. et al. (1994)
Proc. Natl. Acad. Sci. USA
91, 4806-4810.
-
Yamamoto, K. et al. (1994)
Mol. Cell Biol.
14, 4342-4349.
-
Jacobson, N.G. et al. (1995)
J. Exp. Med.
181, 1755-1762.
-
Bacon, C.M. et al. (1995)
Proc. Natl. Acad. Sci. USA
92, 7307-7311.
-
Thierfelder, W.E. et al. (1996)
Nature
382, 171-174.
-
Kaplan, M.H. et al. (1996)
Nature
382, 174-177.
