Neuroscience
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| CSTコード | 包装 | 希望納入価格（円） |
国内在庫  2010年9月9日14時20分 現在 |
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| #2680S | 100 μL | 46,000 | ログインすると国内在庫状況がご確認いただけます。
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| 用途 (希釈倍率) | |
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| ウェスタンブロッティング (1:1,000)、免疫沈降 (1:50)、免疫組織染色 (パラフィン) (1:100)、免疫蛍光染色 (IF-F) (1:200) | |
| 種交差性 | |
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| ヒト、マウス、ラット | |
| 特異性・感度 | |
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| 内在性レベルのp35 タンパク質を検出します。神経毒性傷害の際に、カルパインによって開裂される内在性レベルのp25 タンパク質も検出します。 | |
| 検出タンパク質の分子量 | |
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| 25 kDa、35 kDa | |
| 使用抗原 | |
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| ヒトのp35 タンパク質のC末端領域 (合成ペプチド) | |
| 抗体の由来 | |
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| ウサギ | |
| 貯法 | |
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| -20℃ | |
| 社内データ |
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Western Blotting
Western blot analysis of extracts from mouse brain, rat brain and human cerebellum using p35/p25 (C64B10) Rabbit mAb.
Western Blotting
Western blot analysis of extracts from mouse and rat brain and wild-type mouse and p35 knock-out mouse brain using p35/25 (C64B10) Rabbit mAb (upper) and CDK5 Antibody #2506 (lower). Matching wild-type and p35 knock-out mouse brain was kindly provided by Dr. Li-Huei Tsai, Massachusetts Institute of Technology, Cambridge, MA.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin embedded human meningioma using p35/25 (C64B10) Rabbit mAb #2680.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human astrocytoma using p35/25 (C64B10) Rabbit mAb #2680 in the presence of control peptide (left) or antigen specific peptide (right).
IF-F
Confocal immunofluorescent analysis of cerebellum from normal (left) or p35 knockout (right) mice using p35/25 (C64B10) Rabbit mAb (green). Blue pseudocolor =DRAQ5 ® #4084 (fluorescent DNA dye). Matching wild-type and p35 knock-out mouse brain was kindly provided by Dr. Li-Huei Tsai, Massachusetts Institute of Technology, Cambridge, MA.
| バックグラウンド |
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Cyclin-dependent kinases (CDKs) are serine/threonine kinases that are activated by cyclins and govern eukaryotic cell cycle progression. While CDK5 shares high sequence homology with its family members, it is thought mainly to function in postmitotic neurons, regulating the cytoarchitecture of these cells. Analogous to cyclins, p35 and p39 associate with and activate CDK5 despite the lack of sequence homology. CDK5 is ubiquitously expressed, but high levels of kinase activity are detected primarily in the nervous system due to the narrow expression pattern of p35 and p39 in post-mitotic neurons. A large number of CDK5 substrates have been identified although no discrete substrates have been attributed as a function of p35 vs. p39. Amongst many, substrates of CDK5 include p35 and p39. p35 is rapidly degraded (T1/2 <20 min) by the ubiquitin-proteasome pathway (1). However, p35 stability increases as CDK5 kinase activity decreases, and this is likely a result of decreased phosphorylation of p35 at Thr138 by CDK5 (2). NGF activates Erk and EGR1, and induces p35 expression in PC12 cells (3). Proteolytic cleavage of p35 by calpain produces p25 upon neurotoxic insult, resulting in prolonged activation of CDK5 by p25. Accumulation of p25 is found in neurodegenerative diseases such as Alzheimer's disease and Amyotrophic Lateral Sclerosis (ALS) (4-5).
| 使用例 | |
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本製品は試験研究用です。
