Lymphocyte Signaling
| CSTコード |
包装 |
希望納入価格 (円) |
国内在庫  |
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| #2730S | 100 μL | 46,000 | |
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SHIP2抗体製品一覧
2730 の推奨プロトコール
最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。
注：各製品に最適化されたプロトコールをリンクしています。
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2730:
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Western Blotting
| 用途（希釈倍率） | |
| ウエスタンブロッティング(1:1,000) |
| 特異性・感度 | |
| 内在性レベルのSHIP2 タンパク質を検出します。SHIP1 タンパク質とは交差しません。 |
| 使用抗原 | |
| ヒトのSHIP2 タンパク質のAla1083 周辺領域（合成ペプチド） |
Western Blotting
Western blot analysis of total cell lysates from SKMEL5 and HeLa cells, using SHIP2 Antibody.
SH2-containing inositol phosphatase 1 (SHIP1) is a hematopoietic phosphatase that hydrolyzes phosphatidylinositol-3,4,5-triphosphate to phosphatidylinositol-3,4-bisphosphate (1). SHIP1 is a cytosolic phosphatase with an SH2 domain in its amino terminus and two NPXY Shc binding motifs in its carboxy terminus (1,2). Upon receptor cross-linking, SHIP is first recruited to the membrane junction through binding of its SH2 domain to the phospho-tyrosine in the ITIM motif (2), followed by tyrosine phosphorylation on the NPXY motif (2). The membrane relocalization and phosphorylation on the NPXY motif is essential for the regulatory function of SHIP1 (3-5). Its effect on calcium flux, cell survival, growth, cell cycle arrest and apoptosis is mediated through the PI3K and Akt pathways (3-5). Tyr1021 is located in one of the NPXY motifs in SHIP1, and its phosphorylation is important for SHIP1 function (6).
SHIP2, a homolog of SHIP1, is highly expressed in heart, skeletal muscle and placenta (7). SHIP2 negatively regulates insulin signaling (8) and polymorphisms in SHIP2 have been linked to hyperglycemia (9). Recent studies also suggest SHIP2 as a therapeutic target for the treatment of both obesity and type 2 diabetes (10,11).
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Tridandapani, S. et al. (1997) Mol Cell Biol 17, 4305-11.
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Liu, L. et al. (1997) J Biol Chem 272, 8983-8.
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Malbec, O. et al. (2001) J Biol Chem 276, 30381-91.
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Carver, D.J. et al. (2000) Blood 96, 1449-56.
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Scharenberg, A.M. et al. (1998) EMBO J 17, 1961-72.
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Sattler, M. et al. (2001) J Biol Chem 276, 2451-8.
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Pesesse, X. et al. (1997) Biochem. Biophys. Res. Commun. 239, 697-700.
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Wada, T. et al. (2001) Mol. Cell Biol. 21, 1633-1646.
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Ishida, S. et al. (2006) Pancreas 33, 63-67.
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Dyson, J.M. et al. (2005) Int. J. Biochem. Cell Biol. 37, 2260-2265.
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Sasaoka, T. et al. (2006) Pharmacol Ther 112, 799-809.
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