Protein Folding and Stability
VHL Antibody
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イイネ！(1)
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| CSTコード |
包装 |
希望納入価格 (円) |
国内在庫  |
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| #2738S | 100 μL | 46,000 | |
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2738 の推奨プロトコール
最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。
注：各製品に最適化されたプロトコールをリンクしています。
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2738:
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Western Blotting
| 用途 (希釈倍率) | |
| ウェスタンブロッティング (1:1,000) |
| 特異性・感度 | |
| 内在性レベルのVHL (Isoform 1、2、3) タンパク質を検出します。 |
| 使用抗原 | |
| ヒトのVHL タンパク質のアミノ酸配列 (合成ペプチド) |
| ※括弧付きの動物種は、配列が100%相同であるため反応すると推定されます。 |
Western Blotting
Western blot analysis of lysates from various cell lines using VHL antibody.
The Von Hippel-Lindau (VHL) protein is a substrate recognition component of an E3 ubiquitin ligase complex containing elongin BC (TCEB1 and TCEB2), cullin 1 (CUL1), and RING-box protein 1 (RBX1) (1,2,3). VHL protein has been shown to exist as three distinct isoforms resulting from alternatively spliced transcript variants (4). Loss of VHL protein function results in a dominantly inherited familial cancer syndrome that manifests as angiomas of the retina, hemangioblastomas of the central nervous system, renal clear-cell carcinomas and pheochromocytomas (4). Under normoxic conditions, VHL directs the ubiquitylation and subsequent proteosomal degradation of the hypoxia inducible factor HIF alpha, maintaining very low levels of HIF alpha in the cell. Cellular exposure to hypoxic conditions, or loss of VHL protein function, results in increased HIF alpha protein levels and increased expression of HIF-induced gene products, many of which are angiogenesis factors such as vascular endothelial growth factor (VEGF). Thus, loss of VHL protein function is believed to contribute to the formation of highly vascular neoplasias (4). In addition to HIF alpha, VHL is known to regulate the ubiquitylation of several other proteins, including tat-binding protein 1 (TBP-1), the atypical protein kinase C lambda (aPKC), and two subunits of the multiprotein RNA Polymerase II complex (RPB1 and RPB7) (5,6,7,8). Interactions with elongin BC, RPB1, RPB7 and the pVHL-associated KRAB-A domain containing protein (VHLaK) suggest that VHL may also play a more direct role in transcriptional repression.
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Kibel, A. et al. (1995) Science 269, 1444-6.
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Pause, A. et al. (1997) Proc Natl Acad Sci U S A 94, 2156-61.
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Kamura, T. et al. (2000) Proc Natl Acad Sci U S A 97, 10430-5.
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Czyzyk-Krzeska, M.F. and Meller, J. (2004) Trends Mol Med 10, 146-9.
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Corn, P.G. et al. (2003) Nat Genet 35, 229-37.
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Na, X. et al. (2003) EMBO J 22, 4249-59.
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Kuznetsova, A.V. et al. (2003) Proc Natl Acad Sci U S A 100, 2706-11.
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Li, Z. et al. (2003) EMBO J 22, 1857-67.
PhosphoSitePlus®：
VHL